呼吸氢气对猪心脏缺血再灌注的治疗作用

似乎这是第一篇使用猪为动物疾病模型证明氢气效应的文章。

作者制备两种严重程度不同的模型，第一种是阻断冠状动脉前降支缺血12分钟，再灌注90分钟，第二种延长缺血时间到40分钟，再灌注120分钟。第一种模型研究了呼吸2%氢气102分钟（缺血加再灌注期间）对segment shortening改变的影响，结果发现呼吸2%的氢气可以产生显著的效果（74/48）。第二种模型发现呼吸4%的氢气160分钟，对心脏梗死面积降低的效果比2%氢气（40%）更理想（从对照组的46%降低到治疗后的32%）。研究证明呼吸氢气对猪心脏缺血再灌注效果显著。

从摘要看，本研究缺点是没有进行心脏功能学的研究，既然可以做心电，第二种模型完全可以进行类似的记录。也应该进行心肌酶学的检测。这些都是研究心脏缺血最基本的指标，当然也应该有一些氧化损伤相关指标的研究。这些都没有，说明发表篇论文如此容易，如果只为发表论文，也太糟蹋猪了，也许可以吃肉，废物利用，不知道日本学者是不是这样干的。

Scand Cardiovasc J. 2012 Jan 20. [Epub ahead of print]

Inhalation of hydrogen gas protects against myocardial stunning and infarction in swine.

Sakai K, Cho S, Shibata I, Yoshitomi O, Maekawa T, Sumikawa K.

Abstract

Abstract Objectives: The present study was carried out to determine whether inhalation of hydrogen (H(2)) gas protects myocardium against ischemia-reperfusion (I/R) injury in swine. Design: In anesthetized open-chest swine, myocardial stunning was produced by 12-min occlusion of left anterior descending coronary artery (LAD) followed by 90-min reperfusion in the first study. Group A inhaled 100% oxygen, and group B inhaled 2% H(2) plus 98% oxygen during ischemia and reperfusion. In the second study, myocardial infarction was produced by 40-min occlusion of LAD followed by 120-min reperfusion. Group C inhaled 100% oxygen during ischemia and reperfusion. Group Dinhaled 2% H(2) plus 98% oxygen. Group E inhaled 4% H(2) plus 96% oxygen. Results: The change of segment shortening (%SS) from baseline at 90 min after reperfusion in group B was 74 ± 13 (mean ± SD) %, which was significantly higher than that in group A (48 ± 15%). Myocardial infarct size in group E (32 ± 10%), but not in group D (40 ± 9%) was smaller than that in group C (46 ± 6%). Conclusions: Inhalation of 2% H(2) gas improves myocardial stunning, and inhalation of 4% but not 2% H(2) gas reduces myocardial infarct size in swine.