“拼接体”工作能否再次获得诺贝尔奖？

Spliceosome Structure and Function7.pdf

因拼接体获得诺贝尔奖者：

Phillip Sharp and Richard J. Roberts were awarded the 1993 Nobel Prize in Physiology or Medicine for their discovery of introns and the splicing process

A spliceosome is a large and complex molecular machine found primarily within the splicing speckles of the cell nucleus of eukaryotic cells. The spliceosome is assembled from snRNAs and protein complexes. The spliceosome removes introns from a transcribed pre-mRNA, a kind of primary transcript. This process is generally referred to as splicing.Only eukaryotes have spliceosomes and metazoans have a second spliceosome, the minor spliceosome

拼接体的组装的动态描述（Spliceosome assembly）还没有获奖，可能已经不再值得授予诺贝尔奖

以下是施一公院士的文章，对比上图（已经知道的过程）看看施一公院士的工作究竟有没有新意？

Science. 2015 Aug 20. pii: aac8159. [Epub ahead of print]

Structural basis of pre-mRNA splicing.

Hang J(1), Wan R(1), Yan C(1), Shi Y(2).

Author information:
(1)Ministry of Education Key Laboratory of Protein Science, Tsinghua-Peking Joint
Center for Life Sciences, Center for Structural Biology, School of Life Sciences,
Tsinghua University, Beijing 100084, China. (2)Ministry of Education Key
Laboratory of Protein Science, Tsinghua-Peking Joint Center for Life Sciences,
Center for Structural Biology, School of Life Sciences, Tsinghua University,
Beijing 100084, China. shi-lab@tsinghua.edu.cn.

Splicing of pre-mRNA is performed by the spliceosome. In the cryo-EM structure of
the yeast spliceosome, U5 snRNP acts as a central scaffold onto which U6 and U2
snRNAs are intertwined to form a catalytic center next to Loop I of U5 snRNA.
Magnesium ions are coordinated by conserved nucleotides in U6 snRNA. The intron
lariat is held in place through base pairing interactions with both U2 and U6
snRNAs, leaving the variable-length middle portion on the solvent-accessible
surface of the catalytic center. The protein components of the spliceosome anchor
both 5'- and 3'-ends of the U2 and U6 snRNAs away from the active site, direct
the RNA sequences, and allow sufficient flexibility between the ends and the
catalytic center. Thus, the spliceosome is in essence a protein-directed
ribozyme, with the protein components essential for the delivery of critical RNA
molecules into close proximity of one another at the right time for the splicing
reaction.