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Science：革命性基因编辑技术CRISPR 国际研究进展文献分析

已有 3535 次阅读 2013-8-28 08:27 |个人分类:信息分析|系统分类:科研笔记| 技术, 国际

突然之间，不仅是食品科学家和微生物学家，很多领域都意识到细菌免疫系统的重要性，因为它具备一个非常有价值的特性：以某个特定的基因序列为目标。今年1月，4个研究团队报告了这一被称为 CRISPR 的系统。在接下来的8个月中，许多科研团队利用它来删除、添加、激活或抑制人体、老鼠、斑马鱼、细菌、果蝇、酵母、线虫和农作物细胞中的目标基因，从而证明了这个技术的广泛适用性。

Science：革命性基因编辑技术CRISPR http://www.bio360.net/news/show/6826.html

The CRISPR Craze http://www.sciencemag.org/content/341/6148/833.summary

检索用词 CRISPR

检索时间 2013年8月28日8时

分析平台 http://www.pubmedplus.cn/Pubmed/Statistic

检索结果 PUBMED数据库中检索到相关文献481篇

分析结果如下:

研究主题领域

01. bacteria 248篇 51.559%

02. base sequence 237篇 49.272%

03. repetitive sequences, nucleic acid 203篇 42.204%

04. molecular sequence data 162篇 33.680%

05. dna 142篇 29.522%

06. rna 113篇 23.493%

07. inverted repeat sequences 112篇 23.285%

08. genome, bacterial 101篇 20.998%

09. archaea 89篇 18.503%

10. bacteriophages 87篇 18.087%

11. bacterial proteins 86篇 17.879%

12. genetic variation 85篇 17.672%

13. dna, bacterial 66篇 13.721%

14. escherichia coli 56篇 11.642%

15. sequence analysis, dna 56篇 11.642%

16. mutation 52篇 10.811%

17. rna, bacterial 51篇 10.603%

18. phylogeny 50篇 10.395%

19. dna, intergenic 45篇 9.356%

20. evolution, molecular 44篇 9.148%

21. interspersed repetitive sequences 43篇 8.940%

22. plasmids 41篇 8.524%

23. computational biology 41篇 8.524%

24. models, molecular 40篇 8.316%

25. amino acid sequence 40篇 8.316%

26. recombination, genetic 37篇 7.692%

27. genes, bacterial 36篇 7.484%

28. protein structure, tertiary 34篇 7.069%

29. genome, archaeal 30篇 6.237%

30. models, biological 30篇 6.237%

31. archaeal proteins 29篇 6.029%

32. nucleic acid conformation 29篇 6.029%

33. rna, archaeal 28篇 5.821%

34. crystallography, x-ray 27篇 5.613%

35. genome, viral 24篇 4.990%

36. sequence alignment 23篇 4.782%

37. genetic loci 23篇 4.782%

38. conserved sequence 22篇 4.574%

39. dna, viral 22篇 4.574%

40. cluster analysis 21篇 4.366%

41. escherichia coli proteins 21篇 4.366%

42. genotype 21篇 4.366%

43. bacterial typing techniques 20篇 4.158%

44. gene expression regulation, bacterial 20篇 4.158%

45. multigene family 19篇 3.950%

46. prokaryotic cells 17篇 3.534%

47. rna interference 17篇 3.534%

48. dna, archaeal 16篇 3.326%

49. models, genetic 14篇 2.911%

50. pyrococcus furiosus 13篇 2.703%

文献中的化学物质研究

01. dna, bacterial 66篇 13.721%

02. bacterial proteins 65篇 13.514%

03. rna, bacterial 51篇 10.603%

04. dna, intergenic 42篇 8.732%

05. archaeal proteins 29篇 6.029%

06. rna, archaeal 28篇 5.821%

07. dna, viral 22篇 4.574%

08. escherichia coli proteins 21篇 4.366%

09. rna 19篇 3.950%

10. endoribonucleases 18篇 3.742%

11. dna 16篇 3.326%

12. dna, archaeal 16篇 3.326%

13. dna transposable elements 14篇 2.911%

14. rna, guide 13篇 2.703%

15. deoxyribonucleases 9篇 1.871%

16. rna, small interfering 9篇 1.871%

17. dna primers 9篇 1.871%

18. virulence factors 9篇 1.871%

19. rna, messenger 8篇 1.663%

20. dna-binding proteins 8篇 1.663%

21. dna helicases 7篇 1.455%

22. endonucleases 7篇 1.455%

23. endodeoxyribonucleases 7篇 1.455%

24. ribonucleoproteins 6篇 1.247%

25. macromolecular substances 6篇 1.247%

26. recombinant proteins 6篇 1.247%

27. rna precursors 5篇 1.040%

28. proteins 5篇 1.040%

29. viral proteins 5篇 1.040%

30. rna, untranslated 5篇 1.040%

31. rna-binding proteins 5篇 1.040%

32. bacterial toxins 5篇 1.040%

33. rna, ribosomal, 16s 5篇 1.040%

34. rna, transfer 5篇 1.040%

35. proteome 4篇 0.832%

36. 5' untranslated regions 4篇 0.832%

37. adenosine triphosphate 4篇 0.832%

38. nucleic acids 4篇 0.832%

39. dna restriction-modification enzymes 4篇 0.832%

40. genetic markers 4篇 0.832%

41. h-ns protein, bacteria 3篇 0.624%

42. mutant proteins 3篇 0.624%

43. ribonuclease iii 3篇 0.624%

44. micrornas 3篇 0.624%

45. csy4 endoribonuclease, pseudomonas aeruginosa 3篇 0.624%

46. rna, viral 3篇 0.624%

47. cyclic amp 2篇 0.416%

48. metals 2篇 0.416%

49. eukaryotic initiation factors 2篇 0.416%

50. cyclic amp receptor protein 2篇 0.416%

国际研究机构

01. university of california 28篇 5.821%

02. national institutes of health 13篇 2.703%

03. wageningen university 13篇 2.703%

04. national center for biotechnology information 13篇 2.703%

05. chinese academy of sciences 12篇 2.495%

06. university of copenhagen 12篇 2.495%

07. harvard medical school 11篇 2.287%

08. institut pasteur 11篇 2.287%

09. national library of medicine 10篇 2.079%

10. howard hughes medical institute 10篇 2.079%

11. university of georgia 9篇 1.871%

12. university park 8篇 1.663%

13. tel aviv university 8篇 1.663%

14. montana state university 8篇 1.663%

15. pennsylvania state university 8篇 1.663%

16. the rockefeller university 7篇 1.455%

17. max-planck-institute for terrestrial microbiology 7篇 1.455%

18. vilnius university 7篇 1.455%

19. univ paris-sud 7篇 1.455%

20. northwestern university 6篇 1.247%

21. universite laval 6篇 1.247%

22. university of otago 6篇 1.247%

23. universidad de alicante 6篇 1.247%

24. florida state university 5篇 1.040%

25. cornell university 5篇 1.040%

26. copenhagen university 5篇 1.040%

27. the pennsylvania state university 5篇 1.040%

28. sackler school of medicine 5篇 1.040%

29. thermal biology institute 5篇 1.040%

30. university of illinois 5篇 1.040%

31. university of st andrews 4篇 0.832%

32. harima institute 4篇 0.832%

33. riken spring-8 center 4篇 0.832%

34. kyoto university 4篇 0.832%

35. massachusetts general hospital 4篇 0.832%

36. university of toronto 4篇 0.832%

37. emory university 4篇 0.832%

38. university of vienna 4篇 0.832%

39. felix d'herelle reference center for bacterial viruses 3篇 0.624%

40. dartmouth medical school 3篇 0.624%

41. seoul national university 3篇 0.624%

42. university of washington 3篇 0.624%

43. aix-marseille universite 3篇 0.624%

44. russian academy of sciences 3篇 0.624%

45. waksman institute 3篇 0.624%

46. weizmann institute of science 3篇 0.624%

47. 7 cambridge center 2篇 0.416%

48. azabu university 2篇 0.416%

49. biomedical research institute 2篇 0.416%

50. center for models of life 2篇 0.416%

文献的作者分布

01. Barrangou Rodolphe 28篇 5.821%

02. Brouns Stan J J 19篇 3.950%

03. Doudna Jennifer A 17篇 3.534%

04. Koonin Eugene V 17篇 3.534%

05. Marraffini Luciano A 12篇 2.495%

06. She Qunxin 11篇 2.287%

07. Moineau Sylvain 10篇 2.079%

08. Fineran Peter C 9篇 1.871%

09. Severinov Konstantin 9篇 1.871%

10. Randau Lennart 8篇 1.663%

11. White Malcolm F 7篇 1.455%

12. Prangishvili David 7篇 1.455%

13. Semenova Ekaterina 7篇 1.455%

14. Dudley Edward G 6篇 1.247%

15. Backofen Rolf 6篇 1.247%

16. Gasiunas Giedrius 6篇 1.247%

17. Naismith James H 5篇 1.040%

18. Zhang Feng 5篇 1.040%

19. Lange Sita J 5篇 1.040%

20. Marchfelder Anita 5篇 1.040%

21. Fu Yanfang 4篇 0.832%

22. Reeks Judith 4篇 0.832%

23. Maeder Morgan L 4篇 0.832%

24. Heidrich Nadja 4篇 0.832%

25. Joung J Keith 4篇 0.832%

26. Church George M 4篇 0.832%

27. Jiang Wenyan 4篇 0.832%

28. Hatoum-Aslan Asma 4篇 0.832%

29. Liu Huanting 4篇 0.832%

30. Graham Shirley 4篇 0.832%

31. Zhang Bo 3篇 0.624%

32. Esvelt Kevin M 3篇 0.624%

33. Forterre Patrick 3篇 0.624%

34. Maniv Inbal 3篇 0.624%

35. Sander Jeffry D 3篇 0.624%

36. Reyon Deepak 3篇 0.624%

37. Samai Poulami 3篇 0.624%

38. Shariat Nikki 3篇 0.624%

39. Gersbach Charles A 2篇 0.416%

40. Inanaga Hideko 2篇 0.416%

41. Coppée Jean-Yves 2篇 0.416%

42. Gao Guanjun 2篇 0.416%

43. Llewellyn Anna C 2篇 0.416%

44. Jiao Renjie 2篇 0.416%

45. Mao Yanfei 2篇 0.416%

46. Sampson Timothy R 2篇 0.416%

47. Numata Tomoyuki 2篇 0.416%

48. Osawa Takuo 2篇 0.416%

49. Sezonov Guennadi 2篇 0.416%

50. Saroj Sunil D 2篇 0.416%

文献的国家/地区分布

01. 美国 188篇 39.085%

02. 法国 42篇 8.732%

03. 德国 34篇 7.069%

04. 中国大陆 23篇 4.782%

05. 丹麦 21篇 4.366%

06. 日本 20篇 4.158%

07. 荷兰 18篇 3.742%

08. 加拿大 16篇 3.326%

09. 西班牙 12篇 2.495%

10. 以色列 11篇 2.287%

11. 英国 10篇 2.079%

12. 韩国 9篇 1.871%

13. 新西兰 8篇 1.663%

14. 澳大利亚 8篇 1.663%

15. 立陶宛 7篇 1.455%

16. 奥地利 7篇 1.455%

17. 俄罗斯 6篇 1.247%

18. 瑞士 4篇 0.832%

19. 爱尔兰 4篇 0.832%

20. 瑞典 3篇 0.624%

21. 塞尔维亚 3篇 0.624%

22. 克罗地亚 2篇 0.416%

23. 芬兰 2篇 0.416%

24. 孟加拉国 2篇 0.416%

25. 瓜德罗普岛 1篇 0.208%

26. 巴西 1篇 0.208%

27. 意大利 1篇 0.208%

28. 墨西哥 1篇 0.208%

29. 挪威 1篇 0.208%

30. 波兰 1篇 0.208%

基因本体（细胞组件）

01. host 99篇 20.582%

02. chromosome 36篇 7.484%

03. ribonucleoprotein complex 17篇 3.534%

04. protein complex 8篇 1.663%

05. intracellular 6篇 1.247%

06. virion 4篇 0.832%

07. cell surface 2篇 0.416%

08. vacuole 2篇 0.416%

09. nadh dehydrogenase complex 1篇 0.208%

10. x chromosome 1篇 0.208%

11. cell wall 1篇 0.208%

12. chromosome, telomeric region 1篇 0.208%

13. outer membrane 1篇 0.208%

14. plastid pyruvate dehydrogenase complex 1篇 0.208%

基因本体（分子功能）

01. ethanolamine-phosphate cytidylyltransferase activity 37篇 7.692%

02. dna binding 25篇 5.198%

03. deoxyribodipyrimidine photo-lyase activity 18篇 3.742%

04. rab gtpase binding 8篇 1.663%

05. rna-directed rna polymerase activity 7篇 1.455%

06. metal ion binding 4篇 0.832%

07. double-stranded dna binding 3篇 0.624%

08. pancreatic ribonuclease activity 2篇 0.416%

09. deoxyribonuclease i activity 2篇 0.416%

10. catalytic activity 2篇 0.416%

11. ribosome binding 2篇 0.416%

12. kinase activity 1篇 0.208%

13. atpase activity 1篇 0.208%

14. crotonyl-coa reductase activity 1篇 0.208%

15. ion binding 1篇 0.208%

16. formate dehydrogenase (nad+) activity 1篇 0.208%

17. l-aminoadipate-semialdehyde dehydrogenase activity 1篇 0.208%

18. [acyl-carrier-protein] s-malonyltransferase activity 1篇 0.208%

基因本体（生物过程）

01. developmental process 51篇 10.603%

02. biological regulation 51篇 10.603%

03. transcription, dna-dependent 50篇 10.395%

04. biosynthetic process 49篇 10.187%

05. growth 43篇 8.940%

06. catabolic process 39篇 8.108%

07. rna interference 37篇 7.692%

08. gene silencing 13篇 2.703%

09. translation 7篇 1.455%

10. dna catabolic process 7篇 1.455%

11. innate immune response 6篇 1.247%

12. genetic transfer 5篇 1.040%

13. signal transduction 5篇 1.040%

14. signaling 5篇 1.040%

15. evasion or tolerance of immune response of other organism involved in symbiotic interaction 4篇 0.832%

16. sleep 4篇 0.832%

17. double-strand break repair via homologous recombination 4篇 0.832%

18. localization 3篇 0.624%

19. symbiosis, encompassing mutualism through parasitism 3篇 0.624%

20. adaptive immune response 3篇 0.624%

21. rna catabolic process 3篇 0.624%

22. trna processing 3篇 0.624%

23. chemotaxis 2篇 0.416%

24. behavior 2篇 0.416%

25. carbohydrate utilization 2篇 0.416%

26. cytolysis 2篇 0.416%

27. defense response to virus 2篇 0.416%

28. isotype switching 2篇 0.416%

29. acetate biosynthetic process 1篇 0.208%

30. atp biosynthetic process 1篇 0.208%

31. cofactor biosynthetic process 1篇 0.208%

32. cellular aromatic compound metabolic process 1篇 0.208%

33. chromosome organization 1篇 0.208%

34. dna metabolic process 1篇 0.208%

35. glycolysis 1篇 0.208%

36. fatty acid biosynthetic process 1篇 0.208%

37. lipopolysaccharide biosynthetic process 1篇 0.208%

38. membrane budding 1篇 0.208%

39. lactose metabolic process 1篇 0.208%

40. homeostatic process 1篇 0.208%

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