One key step in the DNA barcoding process   is the establishment of a reliable and comprehensive database (reference   library) that includes both high-quality DNA barcode sequences and accurately   identified voucher specimens (Taberlet, Coissac, Pompanon, Brochmann &   Willerslev, 2012). Once established, such databases can become a valuable   toolkit for molecular ecologists to identify plant material such as pollen,   seeds, seedlings, roots, and even bark fragments (Liu et al., 2018). DNA   barcoding is now becoming essential for a broad range of ecological   applications, such as biodiversity assessments (Lahaye et al., 2008; Yoccoz   et al., 2012), establishing pollination networks (Bell et al., 2016; Vamosi,   Gong, Adamowicz & Packer, 2017), diet analysis (Kartzinel et al., 2015;   Valentini, Pompanon & Taberlet, 2009), and determining the mechanisms   underlying community assembly (Kress et al., 2009, 2010; Pei et al., 2011).

DNA条形码过程中的一个关键步骤是建立一个可靠和全面的数据库（参考库），其中包括高质量的DNA条形码序列和准确鉴定的凭证标本（Taberlet, Coissac, Pompanon, Brochmann & Willerslev, 2012）。一旦建立，这些数据库就可以成为分子生态学家识别植物材料（如花粉、种子、幼苗、根甚至树皮碎片）的宝贵工具（Liu et al., 2018）。DNA条形码现在已成为生态研究多个方面的基础，如生物多样性评估（Lahaye et al., 2008; Yoccoz et al., 2012）、授粉网络建立（Bell et al., 2016; Vamosi, Gong, Adamowicz & Packer, 2017）、食性分析（Kartzinel et al., 2015; Valentini, Pompanon & Taberlet, 2009）和居群构成解析（Kress et al., 2009, 2010; Pei et al., 2011）。