Species Delimitation

Different quantitative methods for   species delimitation were applied, implemented by visual inspection of the   variable nucleotide positions and trees derived from these. These procedures   were conducted only within the independent networks defined in the   statistical parsimony analysis, greatly reducing the complexity of scoring   separated groups. Population profiles of character variation were established   according to Sites and Marshall (2003) as the basis for PAA (Davis and Nixon,   1992) and CHA (Brower, 1999), the latter by assessing variable characters on   the likelihood tree shown below. The WP method also used this tree to delimit   “exclusive” populations, defined as the monophyly of geographically   restricted genotypes to the exclusion of clades elsewhere. Fst values were   used for aggregating samples with non-significant pairwise Fst, in analogy to   the grouping of populations under PAA (Supplementary Fig. S1).

物种划分

采用不同的物种定界定量方法，通过目视检查核苷酸变异位点和由此衍生的树来实现。这些程序仅在简约统计分析中定义的独立网络内进行，大大降低了分组评分的复杂性。根据Sites和Marshall（2003年）建立了特征变异的种群概况，作为PAA（Davis and Nixon,   1992）和CHA（Brower, 1999）的基础，后者通过评估似然树（如下所示）上的变异特征。WP方法还使用这棵树来划分“排他”种群，即局限于一定地地域的基因型的单系，且不包括其他分支。Fst值用于将两两间Fst不显著的样本进行聚集，类似于PAA下的种群分组（附图S1）。