Section.01

常见细胞培养方案

本期推文聚焦 16 种细胞的培养及注意事项！

表 1. 疾病研究常用的实验细胞。

U87MG 细胞

人脑星形胶质母细胞瘤细胞，具有上皮形态，是源自中枢神经系统恶性肿瘤的细胞系。增殖速度快、侵袭性强，还存在明显的遗传与表型异质性^[1]。

培养条件：DMEM 培养基，10% 胎牛血清 FBS，1% 青霉素链霉素双抗，2 mM L-谷氨酰胺^[2]。

图 1. U-87 MG 细胞低密度和高密度生长形态^[3]。

注意事项：

1. 细胞贴壁能力较弱：消化时间短，严格把控时间 (细胞回缩、间隙变大且部分脱落时，加 2-3 mL 完全培养基终止消化)；试剂需充分预热，避免室温放置过久。

2. 易聚集成团：胰酶充分消化并吹散，镜检确认单细胞状态；必要时用多聚赖氨酸包被培养器皿；避免密度过高、血清不适。

3. 血清要求高：选用高品质专用培养基及血清，保障生长速度与贴壁性。

4. 控制接种密度：传代比例 1:2-1:4，70%-80% 密度时传代；密度过低生长缓慢，过高易聚团。

MDA-MB-231 细胞

典型的三阴性乳腺癌 (TNBC) 细胞，具有高侵袭性和转移性^[4]。

培养条件：Leibovitz’s L-15 培养基，10% 胎牛血清，1% 青霉素链霉素双抗^[5]。

图 2. MDA-MB-231 细胞低密度和高密度生长形态^[6]。

注意事项：

1. 接种密度：细胞生长偏慢，传代比例推荐 1:2，避免密度过低。

2. 操作注意：对机械损伤敏感，消化吹打需轻柔、次数少，防止细胞形态异常或生长受阻。

Section.02

小结

不同细胞的培养需求各有差异，本期我们聚焦 16 种常用细胞，整理分享了实操性极强的培养经验。希望这些干货内容能切实帮到大家，让细胞培养更顺利、更高效！

产品推荐

特级胎牛血清，乌拉圭

MCE 特级胎牛血清 (HY-T1000) 来源于非疫区健康牛 (不含 BVDV、PI3、IBR、BTV 等牛源病毒)，由剖腹产 5-8 月龄胎牛的血液制备而成，产地乌拉圭。

高糖 DMEM 培养基，含 L-谷氨酰胺、丙酮酸钠和酚红，不含 HEPES

DMEM (HY-K3001) 是广泛使用的基础培养基，适于培养多种哺乳动物细胞，包括 Hela、293、Cos-7、PC-12 等细胞系，以及原代成纤维细胞、神经元、神经胶质细胞、人脐带静脉内皮细胞、平滑肌细胞等。

DMEM/F-12 (1:1) 培养基，含 L-谷氨酰胺、酚红和 HEPES

DMEM/F-12 (HY-K3002) 是广泛使用的基础培养基，适于培养多种哺乳动物细胞，包括 MDCK、神经胶质细胞、成纤维细胞和人内皮细胞等。

低糖 DMEM 培养基，含 L-谷氨酰胺，丙酮酸钠和酚红，不含 HEPES

DMEM (HY-K3003) 是广泛使用的基础培养基，适于培养多种哺乳动物细胞，包括 Hela、293、Cos-7、PC-12 等细胞系，以及原代成纤维细胞、神经元、神经胶质细胞、人脐带静脉内皮细胞、平滑肌细胞等。

RPMI 1640 培养基，含 L-谷氨酰胺和酚红，不含丙酮酸钠和HEPES

RPMI 1640培养基 (HY-K3004) 含有还原型谷胱甘肽和高浓度的维生素，且含 MEM 和 DMEM 中没有的生物素、维生素 B12 和对氨基苯甲酸 (PABA)，以及高浓度的肌醇和氯化胆碱。

Mouse CD3/CD28 T Cell Activation Magnetic Beads

Mouse CD3/CD28 T Cell Activation Magnetic Beads (HY-K0354) 基于 CD3 和 CD28 两种重要的共刺激信号，无需依赖饲养层细胞 (抗原递呈细胞) 或抗原即可简单快速地激活 T 细胞。

MEM 非必需氨基酸溶液 (100X)

MEM 非必需氨基酸溶液 (100×) (HY-K3011)，是已经过滤除菌，可直接使用的常用细胞培养添加剂，其主要促进细胞生长和存活，减少体外细胞的生物合成负担。

原文参考：https://mp.weixin.qq.com/s/_s6_rIY-FNreHpTW0cGo-w

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