The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

First author: Haijun Zhao; Affiliations: USDA ARS Dale Bumpers National Rice Research Center (美国农业部国家水稻研究中心): Stuttgart, USA

Corresponding author: Yulin Jia

Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical (非典型的) resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance (广谱稻瘟病抗性) mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm (细胞质). A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.

植物抗性基因（R基因）通常编码一个含核酸结合位点-富含亮氨酸重复（NLR）结构域的蛋白。本文鉴定了水稻中的一个非典型R基因Ptr，其编码一个包含4个Armadillo重复的蛋白。Ptr基因作用于由R基因Pi-ta及其关联基因Pi-ta2介导的水稻稻瘟病广谱抗性。Ptr基因为组成型表达，编码两个异构体，主要定位于细胞质中。快速中子诱变株系M2354中Ptr基因编码区的一对碱基缺失造成蛋白被截短，导致水易感稻瘟病菌的侵染。利用CRISPR/Cas9技术定向突变抗性植株中的Ptr基因同样能够增加水稻对于稻瘟病菌的易感性，进一步验证了该基因的功能。对于Ptr基因的克隆将有助于研究水稻对稻瘟病菌的广谱抗性。

通讯：Yulin Jia  (https://www.ars.usda.gov/people-locations/person?person-id=21002)

研究方向：植物病理学。

doi: https://doi.org/10.1038/s41467-018-04369-4

Journal: Nature Communications

First Published: 23 May, 2018

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