第三,图表的标注
也应该独立成文,并且无需阅读全文就能完全理解。因此,缩写要么不用,要么先定义后才用,同时应该避免使用技术术语。标注应清楚说明做了什么、观察到了什
么;也应简述使用了什么统计检验,给出P值,并对所用的符号进行定义。除了其中描述方法的语句,标注和图表标题都应采用现在时。比如,应该
写:“Western blot showing an increase in the levels of p53
after…”而不是“Western blotting showed that the levels of p53 increased
after…”。
英文原文 Display items: a picture tells a thousand words
In
the chapter on abstracts I mentioned that many readers will only read
the abstract of your paper, and so the abstract needed to be
self-contained, describing all of the important findings and their
significance. Some readers will go further than just the abstract and
look at the display items to validate the findings described in the
abstract, but still not read the entire paper. Thus, like the abstract,
the display items in your paper (along with their associated legends)
need to be able to stand alone and be understood without the need to
refer to the text of the paper.
Display items include figures
and tables, which are essentially graphical representations of the
results described in the text. Simply put, they are the most effective
and efficient way to present your results. With good figures and tables
you will be able to impart to the reader exactly what you found in your
study in a relatively short period of time (that is, much faster than
it would take the reader to read the entire paper). Researchers in
rapidly moving fields or with limited time to keep up to date with
advances in their field will appreciate the rapidity with which they
can be informed of your findings; journals editors and peer reviewers
will appreciate the clarity. Therefore, it is worthwhile devoting some
thought and attention to developing good quality figures and tables
that clearly convey your results. As with the other sections of
scientific manuscripts, there are certain ‘rules’ that should be
followed when generating display items. First, if you have a target
journal in mind already, consider how many display items they allow and
ensure that you do not exceed that limit. If you have more results to
describe than can be simply shown in the allowable number of display
items, some may need to be included in a Supplementary Information
section, or described in the text with the statement “(data not
shown)”. However, only the least important or peripheral findings
should be described in this way, and all findings that support your
hypothesis need to be shown. Thus, you may need to consider an
alternative journal if your first choice will not allow you to present
all of your important data. By contrast, if a journal allows more
display items than is necessary to show your findings, do not add
redundant or unnecessary display items simply because you can. All
display items must have a clear and necessary purpose.
Second,
the data shown in figures and tables needs to be easy to interpret.
Consider how much data you wish to show in a given display item and how
it can be organized to convey the important message. Therefore, rather
than combining multiple parameters or treatments into a single graphic,
consider splitting the data across multiple simpler graphics that can
be grouped together in a single figure. Remember to clearly label any
graph axes, table columns and rows, and components of diagrams if
appropriate. Trendlines, scale bars and the results of any statistical
tests should be also shown, where relevant, for example by using an
asterisk to indicate significance, or a variety of symbols to indicate
different levels of significance. With large samples, report the %
change or % difference as well as absolute values. Third, the
legends accompanying display items need to be able to stand alone such
that the display items are entirely understandable without the need to
read the entire manuscript. That means abbreviations should not be used
or need to be defined, and technical terms should be avoided. It should
be clear exactly what was done and what was seen. Statistical tests
should be briefly described in the legends, with p values given and any
symbols used defined. Legends, including their headings, should be
written in the present tense with the exception of any methods
described within them. For example, use “Western blot showing an
increase in the levels of p53 after…” rather than “Western blotting
showed that the levels of p53 increased after…”.
Finally, as
mentioned in the chapter on writing the results section, there should
be no redundancies between the display items and the text. Therefore,
do not produce a display item to show information that can easily and
briefly be stated in the text, and do not duplicate information among
tables and figures, for example, by making a table to show the same
information already conveyed in a figure. Do not embed figures and
their legends within the text of the manuscript you plan to submit. The
publisher will put your text and display items into a template ‘proof’
that will be specific to the target journal. What they usually require
from authors is a separate file containing display items (occasionally
these can be placed at the end of a manuscript files) and a text file
that includes figure and table legends listed together at the end
(usually following the references). Check the instructions for authors
of your intended target journal for their specific requirements.
By
preparing good quality, clear display items before writing the results
section, this section will practically write itself. The display items
can be grouped in a logical order that progresses your argument or
progressively strengthens your hypothesis. With one subsection and one
display item for each of the major findings, the subsection headings
will be similar to the relevant legend headings, and the text in each
subsection will provide a brief description of the findings shown in
each display item, complete with the results of statistical analyses,
with the reader being referred to the display items for more detail.
Examples
Tables
are a great way to present large amounts of necessary data with minimal
description required. The table shown above is a truncated version of a
table in a paper published in The Journal of Clinical Investigation
(doi:10.1172/JCI37622; reproduced with permission). The data presented
clearly and economically in this table would have required a
considerable amount of word space to describe in the text, but the use
of a table makes the information available without the need for a wordy
description. All that was required to describe this in the main text
was the following statement: “Clinical characteristics of all patients
and tumor samples are summarized in Table 1”. The figure below, taken
from the same paper, contains many of the elements of a successful
display item described in this chapter and listed in the checklist
below.
Checklist 1. ‘Stand alone’ legends 2. Comply with the allowable number of display items 3. Avoid redundancy among display items or between display items and text 4. Divide data showing different effects or parameters among different panels within the same display item 5. Use scale bars, trend lines and clear labels, and show the results of statistical tests 6. Avoid or define all symbols and abbreviations 7. With large samples, show % changes/differences as well as absolute values 8. Submit figures in a separate file or at the end of the manuscript file rather than embedded in the main text 9.
Check the instructions for authors for any specific requirements
regarding format, size, color, number of items and any other parameters