Klf7 modulates the differentiation and proliferation of chicken preadipocyte

Zhiwei Zhang, Haixia Wang, Yingning Sun, Hui Li and Ning Wang

Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China  

Acta Biochim Biophys Sin 2013, 45: 280–288; doi: 10.1093/abbs/gmt010

Krüppel-like factor 7 (Klf7) has been extensively studied in the mammalian species, but its function in avian species is unclear. The objective of this study was to reveal the function of chicken Klf7 (Gallus gallus Klf7, gKlf7) in adipogenesis. The results of real-time reverse transcription polymerase chain reaction demonstrated that the relative mRNA level of chicken Klf7 (gKlf7/gβ-Actin) in the abdominal adipose tissue was significantly associated with the abdominal fat content and the age of broilers (P < 0.05), and gKlf7 was more highly expressed in preadipocytes than in mature adipocytes (P < 0.05). In addition, Oil red O staining showed that gKlf7 inhibited chicken preadipocyte differentiation, and MTT assay indicated that gKlf7 overexpression promoted preadipocyte proliferation. Additionally, luciferase assays showed that gKlf7 overexpression suppressed the chicken CCAAT/enhancer-binding protein α (C/ebpα), fatty acid synthase (Fasn), and lipoprotein lipase (Lpl) promoter activities (P < 0.05), and gKlf7 knockdown increased the chicken peroxisome proliferator-activated receptor γ (Pparγ), C/ebpα and fatty acid-binding protein 4 (Fabp4) promoter activities (P < 0.05). Together, our study demonstrated that chicken Klf7 inhibits preadipocyte differentiation and promotes preadipocyte proliferation.

Effects of Klf7-knockdown and Klf7-overexpression on chicken preadipocyte differentiation

全文: http://abbs.oxfordjournals.org/content/45/4/280.full.pdf+html 

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