Anti-inflammatory effect of resveratrol through the suppression of NF-κB and JAK/STAT signaling pathways

Chunfang Ma,  Yin Wang,  Lei Dong,  Minjing Li and  Wanru Cai
Acta Biochim Biophys Sin 2015, 47: 207–213; doi: 10.1093/abbs/gmu135

The Second Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310005, China

Resveratrol, the most important ingredient extracted from Polygonum cuspidatum, exerts cytoprotective effects via anti-inflammatory actions in vitro. In this study, we investigated this effect of resveratrol on the lipopolysaccharide (LPS)-induced inflammatory response and its underlying molecular mechanism of action in RAW264.7 murine macrophages. Results showed that resveratrol down-regulated the expression of inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6), therefore, suppressed the production of nitric oxide and the secretion of IL-6 in LPS-stimulated RAW264.7 cells in a dose-dependent manner. Resveratrol also inhibited the translocation of high-mobility group box 1 (HMGB1) from the nucleus to the cytoplasm and of nuclear transcription factor kappa-B (NF-κB) p65 from the cytoplasm to the nucleus; it suppressed the phosphorylation of IκBα. Furthermore, these actions were mediated by suppressing the phosphorylation of signal transducer and activator of transcription (STAT)-1 and -3. In conclusion, these data indicate that resveratrol exerts anti-inflammatory effects, at least in part by reducing the release of HMGB1 and modulating the NF-κB and Janus kinase/STAT signaling pathways. Resveratrol could potentially be developed as a useful agent for the chemoprevention of inflammatory diseases.

图例: HMGB1在细胞质和细胞核中的分布

全文: http://abbs.oxfordjournals.org/content/47/3/207.full.pdf+html