HISTONE DEACETYLASE 9 stimulates auxin-dependent thermomorphogenesis in Arabidopsis thaliana by mediating H2A.Z depletion

First author: Lennard C. van der Woude; Affiliations: Utrecht University (乌特勒支大学): Utrecht, Netherlands

Corresponding author: Martijn van Zanten

Many plant species respond to unfavorable high ambient temperatures by adjusting their vegetative body plan to facilitate cooling. This process is known as thermomorphogenesis and is induced by the phytohormone auxin. Here, we demonstrate that the chromatin-modifying enzyme HISTONE DEACETYLASE 9 (HDA9) mediates thermomorphogenesis but does not interfere with hypocotyl elongation during shade avoidance. HDA9 is stabilized in response to high temperature and mediates histone deacetylation at the YUCCA8 locus, a rate-limiting enzyme in auxin biosynthesis, at warm temperatures. We show that HDA9 permits net eviction of the H2A.Z histone variant from nucleosomes associated with YUCCA8, allowing binding and transcriptional activation by PHYTOCHROME INTERACTING FACTOR 4, followed by auxin accumulation and thermomorphogenesis.

许多植物会通过调整其营养器官体轴来降低自身的温度，从而适应不适宜的高温环境。该过程叫做植物的热形态建成，是由植物激素生长素所诱导的。本文中，作者揭示了染色质修饰酶HDA9介导了植物的热形态建成，但并不影响避荫过程中下胚轴的伸长。HDA9蛋白在响应高温时比较稳定，在气温温暖时介导生长素合成途径中的一个限速酶YUCCA8位点的组蛋白去乙酰化。作者的研究显示HDA9会将H2A.Z组蛋白变体驱逐出YUCCA8相关的核小体，从而使得光敏色素互作因子PIF4能够顺利结合并激活YUCCA8基因的转录，从而促进下游的生长素积累和热形态建成。

通讯：Martijn van Zanten（https://www.uu.nl/en/research/molecular-plant-physiology/dr-martijn-van-zanten）

个人简介：2004-2009年，乌特勒支大学，博士；2009-2011年，马克斯·普朗克植物育种研究所，博士后。

研究方向：植物热形态建成背后潜在的分子遗传调控机制。

doi: https://doi.org/10.1073/pnas.1911694116

Journal: PNAS

First Published: November 25, 2019