A SOSEKI-based coordinate system interprets global polarity cues in Arabidopsis

First author: Saiko Yoshida; Affiliations: Wageningen University (瓦赫宁根大学): Wageningen, The Netherlands

Corresponding author: Dolf Weijers

Multicellular development requires coordinated cell polarization relative to body axes, and translation to oriented cell division. In plants, it is unknown how cell polarities are connected to organismal axes and translated to division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal and radial organismal axes to localize to polar cell edges. Localization does not depend on tissue context, requires cell wall integrity and is defined by a transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity regulator. The DIX-like domain self-interacts and is required for edge localization and for influencing division orientation, together with a second domain that defines the polar membrane domain. Our work shows that SOSEKI proteins locally interpret global polarity cues and can influence cell division orientation. Furthermore, this work reveals that, despite fundamental differences, cell polarity mechanisms in plants and animals converge on a similar protein domain.

多细胞的发育需要协调细胞相对于体轴的极性，进一步指导细胞分裂的方向。在植物中，细胞极性是如何与植株体轴及细胞分裂方向关联起来的还不清楚。本文中，作者鉴定到了拟南芥SOSEKI蛋白通过整合植株顶-基轴和径向轴来定位极性细胞的边缘。这种定位并不依赖于组织类型，需要细胞壁完整，并且由一个可移动的蛋白特异性基序所决定。SOSEKI中的一个未知功能结构域DUF与动物Dishevelled极性调控子的DIX齐聚结构域类似。该类DIX结构域能够自互作，并且与另外一个定义极性膜结构域一起是极性细胞边缘定位和影响细胞分裂方向所必需的。本文的工作显示SOSEKI蛋白能够局部解释整体极性，并影响细胞分裂的方向。此外，本文的研究结果揭示了植物和动物有关细胞极性除了基本的一些差异外，二者之间存在类似的蛋白结构域。

通讯：Dolf Weijers (https://f1000.com/prime/thefaculty/member/1058938112246895)

个人简介：1997年，荷兰恩斯赫德，生物化学学士；2002年，荷兰莱顿大学，发育遗传学博士；2002-2006年，德国图宾根大学，Gerd Jürgens实验室博士后。

研究方向：以拟南芥早期胚胎会材料研究细胞如何获取其特性。

doi: https://doi.org/10.1038/s41477-019-0363-6

Journal: Nature Plants

Published date: February 08, 2019