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ABBS: miR-145 targets vascular endothelial growth facto

已有 2193 次阅读 2014-11-14 08:46 |个人分类:期刊新闻|系统分类:论文交流| China, Growth

MicroRNA-145 targets vascular endothelial growth factor and inhibits invasion and metastasis of osteosarcoma cells

Lei Fan, Qiang Wu, Xiaojuan Xing, Yulong Wei, and Zengwu Shao

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

MicroRNAs are important gene regulators that play a profound role in tumorigenesis. MicroRNA-145 (miR-145), an important member in the family of microRNAs, is under-expressed in several types of tumors and acts as a tumor suppressor. The role and probable pathways of miR-145 in osteosarcoma carcinogenesis are still unknown. In this study, we found that miR-145 was significantly under-expressed in osteosarcoma tissues, and the over-expression of miR-145 could inhibit invasion and angiopoiesis of osteosarcoma cells. Furthermore, the results showed that vascular endothelial growth factor (VEGF) expression was down-regulated in osteosarcoma cells after miR-145 transfection. On the basis of these results, we performed the luciferase assay and verified that miR-145 could down-regulate VEGF at the translational level by partially binding to VEGF 3 untranslated region (3UTR). Therefore, it can be concluded that miR-145 can inhibit invasion and metastasis of osteosarcoma cells. One of the mechanisms is the down-regulation of VEGF expression by miR-145 by binding to the 3UTR of VEGF mRNA specifically. These novel findings may have extensive implications for an effective gene therapy of osteosarcoma.

图例: miR-145对细胞侵染的影响

Acta Biochim Biophys Sin (Shanghai). 2012 May;44(5):407-14. doi: 10.1093/abbs/gms019

全文: http://abbs.oxfordjournals.org/content/44/5/407.full.pdf+html

引用此文的文献:

1 Changes in microRNA expression in the MG-63 osteosarcoma cell line compared with osteoblasts

2 MicroRNA-145 downregulation associates with advanced tumor progression and poor prognosis in patients suffering osteosarcoma

3 miR-16 inhibits cell proliferation by targeting IGF1R and the Raf1-MEK1/2-ERK1/2 pathway in osteosarcoma

4 Diallyl trisulfide inhibits proliferation, invasion and angiogenesis of osteosarcoma cells by switching on suppressor microRNAs and inactivating of Notch-1 signaling

5 MicroRNAs in osteosarcoma: diagnostic and therapeutic aspects

6 Loss of microRNA-132 predicts poor prognosis in patients with primary osteosarcoma

7 VEGF-A mRNA processing, stability and translation: a paradigm for intricate regulation of gene expression at the post-transcriptional level

8 MicroRNAs in Osteosarcomagenesis

9 Substrate-Mediated Delivery of MicroRNA-145 Through a Polysorbitol-Based Osmotically Active Transporter Suppresses Smooth Muscle Cell Proliferation: Implications for Restenosis Treatment

10 Tumor-Suppressing Effects of miR451 in Human Osteosarcoma

11 Liposarcoma miRNA signatures identified from genome-wide miRNA expression profiling

12 microRNA-145 inhibits osteosarcoma cell proliferation and invasion by targeting ROCK1

13 MicroRNA-101 inhibits the metastasis of osteosarcoma cells by downregulation of EZH2 expression



https://blog.sciencenet.cn/blog-592748-843311.html

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