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MicroRNA-145 targets vascular endothelial growth factor and inhibits invasion and metastasis of osteosarcoma cells
Lei Fan, Qiang Wu, Xiaojuan Xing, Yulong Wei, and Zengwu Shao
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
MicroRNAs are important gene regulators that play a profound role in tumorigenesis. MicroRNA-145 (miR-145), an important member in the family of microRNAs, is under-expressed in several types of tumors and acts as a tumor suppressor. The role and probable pathways of miR-145 in osteosarcoma carcinogenesis are still unknown. In this study, we found that miR-145 was significantly under-expressed in osteosarcoma tissues, and the over-expression of miR-145 could inhibit invasion and angiopoiesis of osteosarcoma cells. Furthermore, the results showed that vascular endothelial growth factor (VEGF) expression was down-regulated in osteosarcoma cells after miR-145 transfection. On the basis of these results, we performed the luciferase assay and verified that miR-145 could down-regulate VEGF at the translational level by partially binding to VEGF 3 untranslated region (3UTR). Therefore, it can be concluded that miR-145 can inhibit invasion and metastasis of osteosarcoma cells. One of the mechanisms is the down-regulation of VEGF expression by miR-145 by binding to the 3UTR of VEGF mRNA specifically. These novel findings may have extensive implications for an effective gene therapy of osteosarcoma.
图例: miR-145对细胞侵染的影响
Acta Biochim Biophys Sin (Shanghai). 2012 May;44(5):407-14. doi: 10.1093/abbs/gms019
全文: http://abbs.oxfordjournals.org/content/44/5/407.full.pdf+html
引用此文的文献:
1 Changes in microRNA expression in the MG-63 osteosarcoma cell line compared with osteoblasts
5 MicroRNAs in osteosarcoma: diagnostic and therapeutic aspects
6 Loss of microRNA-132 predicts poor prognosis in patients with primary osteosarcoma
8 MicroRNAs in Osteosarcomagenesis
10 Tumor-Suppressing Effects of miR451 in Human Osteosarcoma
11 Liposarcoma miRNA signatures identified from genome-wide miRNA expression profiling
12 microRNA-145 inhibits osteosarcoma cell proliferation and invasion by targeting ROCK1
13 MicroRNA-101 inhibits the metastasis of osteosarcoma cells by downregulation of EZH2 expression
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