Proteomic analysis of BmN cells (Bombyx mori) in response to infection with Nosema bombycis

Xinyi He, Xiangkang He, Han Liu, Mingqian Li, Shunfeng Cai, Zhangwuke Fu and Xingmeng Lu

Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Nosemabombycis (N. bombycis, Nb) is an obligate intracellular parasite, which can cause pebrine disease in the silkworm. To investigate the effects of N. bombycisinfection on the host cells, proteomes from BmNcells that had or had not been infected with N. bombycis at different infection stages were characterized with two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry, which identified 24 differentially expressed host proteins with significant intensity differences (P < 0.05) at least at one time point in mock- and N. bombycis infected cells. Notably, gene ontology analyses showed that these proteins are involved in many important biological reactions. During the infection phase, proteins involved in energy metabolism and oxidative stress had up-regulated expression. Two proteins participated in ubiquitin-dependent protein catabolic process had down-regulated expression. Quantitative real-time polymerase chain reaction was used to analyze the transcriptional profiles of these identified proteins. Taken together, the abundance changes, putative functions, and participation in biological reactions for the identified proteins produce a host-responsive protein model in N. bombycis-infected BmNcells. These findings further our knowledge about the effect of energy defect parasites on the host cells.

图例: 家蚕微孢子虫感染宿主细胞

Acta Biochim Biophys Sin (Shanghai). 2014 Nov;46(11):982-90. doi: 10.1093/abbs/gmu092

全文: http://abbs.oxfordjournals.org/content/46/11/982.full

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