Distinct transcription profile of genes involved in carotenoid biosynthesis among six different color carrot (Daucus carota L.) cultivars

Jing Ma, Zhisheng Xu, Guofei Tan, Feng Wang, and Aisheng Xiong

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Acta Biochim Biophys Sin 2017, 49: 817–826; doi: 10.1093/abbs/gmx081

Carotenoid, a group of lipophilic molecules, is widely distributed in nature, and is important for plant photosynthesis and photoprotection. In carrot taproot, different types of dominant carotenoid accumulation lead to yellow, orange, and red colors. In this study, six different carrot cultivars were used to simultaneously analyze carotenoid contents by high performance liquid chromatography. The expression levels of genes involved in carotenoid biosynthesis of carrot were also detected by real-time quantitative PCR. It was found that genes involved in xanthophyll formation were expressed at high levels in yellow carrot cultivars. However, these genes were expressed at low levels in orange carrot cultivars. The contents of α- and β-carotene accounted for a large proportion in total carotenoid contents in orange carrot cultivars. These results indicate that α-carotene accumulation and xanthophyll formation may be related to the expression levels of carotene hydroxylase genes in carrot.

Growth status and transverse section of different cultivars of carrot taproots

阅读原文: http://www.abbs.org.cn/arts.asp?id=4203

获取全文: abbs@sibs.ac.cn

相关论文:

1 Carotenoid Metabolism in Plants

2 Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins

3 Regulation of Carotenoid Metabolism in Tomato

4 Illuminating colors: regulation of carotenoid biosynthesis and accumulation by light

5 Comparative analysis of pigments in red and yellow banana fruit

6 Carotenoid gene expression explains the difference of carotenoid accumulation in carrot root tissues

7 A MYB transcription factor, DcMYB6, is involved in regulating anthocyanin biosynthesis in purple carrot taproots

8 Fast, cross cultivar determination of total carotenoids in intact carrot tissue by Raman spectroscopy and Partial Least Squares calibration