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2024年8月6日,Elsevier 旗下国际著名期刊《Biotechnology Advances》在线发表了云南师范大学生命科学学院丁俊美教授课题组的综述类研究成果《Yeast surface display technology: Mechanisms, applications, and perspectives》。
云南师范大学生命科学学院丁俊美教授为通讯作者。
Microbial cell surface display technology, which relies on genetically fusing heterologous target proteins to the cell wall through fusion with cell wall anchor proteins, has emerged as a promising and powerful method with diverse applications in biotechnology and biomedicine. Compared to classical intracellular or extracellular expression (secretion) systems, the cell surface display strategy stands out by eliminating the necessity for enzyme purification, overcoming substrate transport limitations, and demonstrating enhanced activity, stability, and selectivity. Unlike phage or bacterial surface display, the yeast surface display (YSD) system offers distinct advantages, including its large cell size, ease of culture and genetic manipulation, the use of generally regarded as safe (GRAS) host cell, the ability to ensure correct folding of complex eukaryotic proteins, and the potential for post-translational modifications. To date, YSD systems have found widespread applications in protein engineering, waste biorefineries, bioremediation, and the production of biocatalysts and biosensors. This review focuses on detailing various strategies and mechanisms for constructing YSD systems, providing a comprehensive overview of both fundamental principles and practical applications. Finally, the review outlines future perspectives for developing novel forms of YSD systems and explores potential applications in diverse fields.
KeywordsYeast surface displayWhole cell biocatalystScaffoldinSaccharomyces cerevisiaeAnchor proteinBioremediation
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丁俊美-云南师范大学生命科学学院
云南师范大学生命科学学院研究生导师简况表
姓名:丁俊美 职称:教授、博士生导师 E-mail:djm@ynnu.edu.cn, djm3417@163.com
教育经历(Education): 2002/09-2006/07 鲁东大学 生物科学 学士 2006/09-2012/07 云南大学 微生物学 博士 | |
工作经历(Employments): 2020/11-至今 云南师范大学 教授、博导 2015/10-2020/11 云南师范大学 副教授、硕导 2018/01-2019/01 国家自然科学基金委员会 生命科学学部 兼聘教师 2015/09-2016/09 美国麻省理工学院(Koch Institute) 访问学者 2012/09-2015/10 云南师范大学 讲师
学术任职(Education): 中国环境微生物学会委员
研究方向(Research Interests): 1. 环境污染物生物降解; 2. 极端生境微生物资源及酶的挖掘; 3. 新型CRISPR/Cas系统;
科研、人才项目(Grants): 1. 国家自然科学基金面上项目:腾冲脂环酸芽孢杆菌D-1中AtCas9的结构特征及作用机制研究(2021.01-2024.12); 2. 国家自然科学基金地区项目:腾冲脂环酸芽孢杆菌D-1中新型CRISPR/Cas9系统作用机制研究(2020.01-2023.12); 3. 国家自然科学基金青年项目:GDSL酯酶的定向进化与分子改造研究(2017.01-2019.12); 4. 国家自然科学基金地区项目:Asr1p在酿酒酵母细胞周期G2/M转变中的作用机制(2015.01-2018.12); 5. 云南省自然科学基金面上项目:羧酸酯酶表面展示型全细胞催化剂构建及其对增塑剂邻苯二甲酸酯降解的功能研究(2016.10-2019.12); 6. 云南省中青年学术和技术带头人后备人才(2018HB05,2018.01-2022.12); 7. 云南省“万人计划”青年拔尖人才(YNWR-QNBJ-2019-001,2020.01-2024.12);
近三年代表性论文(Papers): 1. Liu Y, Wang X, Nong S, Bai Z, Han N, Wu Q, Huang Z, Ding J*. Display of a novel carboxylesterase CarCby on Escherichia coli cell surface for carbaryl pesticide bioremediation. Microb Cell Fact. 2022, 21:97. 2. Ding J#*, Liu Y#, Gao Y, Zhang C, Wang Y, Xu B, Yang Y, Wu Q, Huang Z*. Biodegradation of λ-cyhalothrin through cell surface display of bacterial carboxylesterase. Chemosphere. 2022, 289:133130. 3. Yu Y, Yang J, Teng Z, Zheng L, Sheng Q, Li P, Fu H, Li C, Chen Y, Zhang Y, Ding J*, Chen X*. D-Alanine metabolism via d-Ala aminotransferase by a marine Gammaproteobacterium, Pseudoalteromonas sp. strain CF6-2. Appl Environ Microbiol. 2022, 88:e0221921. 4. Li J, Cheng J, Teng Z, Zhang X, Chen X, Sun M, Wang J, Zhang Y, Ding J*, Tian X*, Zhang X*. A novel gelatinase from marine flocculibacter collagenilyticus SM1988: characterization and potential application in collagen oligopeptide-rich hydrolysate preparation. Mar Drugs. 2022, 20:48. 5. Gao C, Zhang N, He X, Wang N, Zhang X, Wang P, Chen X, Yu-Zhong Zhang Y, Ding J*, Li C*. Characterization of the trimethylamine N-oxide transporter from Pelagibacter Strain HTCC1062 reveals its oligotrophic niche adaption. Front Microbiol. 2022, 13:838608. 6. Cheng J, Zhang X, Wang Z, Zhang X, Liu S, Song X, Zhang Y, Ding J*, Chen X*, Xu F*. Potential of thermolysin-like protease A69 in preparation of bovine collagen peptides with moisture-retention ability and antioxidative Activity. Mar Drugs. 2021, 19:676. 7. Ding J#*, Zhou Y#, Wang C, Peng Z, Mu Y, Tang X, Huang Z*. 2020. Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli. Microb Cell Fact. 2020, 19:114. 8. Ding J#*, Zhou Y#, Zhu H, Deng M, Gao Y, Yang Y, Huang Z*. Characterization of EstZY: A new acetylesterase with 7-aminocephalosporanic acid deacetylase activity from Alicyclobacillus tengchongensis. Int J Biol Macromol. 2020, 148:333-341.
近三年主讲课程(Courses): 本科生课程:《微生物学》、《微生物学实验》; 研究生课程:《基因编辑技术》、《改变生活的生物技术》。 |
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