2024年3月22日，Elsevier 旗下国际著名期刊《Journal of Environmental Management》在线发表了云南师范大学能源与环境科学学院梁承月博士与南京工程学院合作的最新研究成果《Decolorization and detoxification of Brilliant Crocein GR by a newly enriched thermophilic consortium》。云南师范大学能源与环境科学学院梁承月为通讯作者。

Abstract

The environmental pollution caused by azo dyes at high temperatures has become an urgent problem. However, little attention has been paid to decolorizing azo dyes by thermophilic consortiums. In this study, a thermophilic bacterial consortium (BCGR-T) mainly composed of two genera, namely, Caldibacillus (70.90%) and Aeribacillus (17.63%) was first enriched, which can decolorize Brilliant Crocein GR (BCGR) at high temperatures (50–75 °C), pH values of 6∼8, dye concentrations (100–400 mg/L) and salinities (1–5%, w/v). The enzyme activity results showed that the azoreductase activity was nearly 8.8 times that of the control (p < 0.01), and the intracellular lignin peroxidase was also highly expressed with enzyme activity of 5.64 U (min−1 mg−1 protein) (p < 0.05), indicated that both azoreductase and intracellular lignin peroxidase played an important part in the decolorization process. Furthermore, seven new intermediate metabolic products, including aniline, phthalic acid, 2-carboxy benzaldehyde, phenylacetic acid, benzoic acid, toluene, and 4-methyl-hexanoic acid, were identified. In addition, functional genes related with the azo dye decolorization, such as those encoding the azoreductase, laccase, FMN reductase, NADPH-/NADH-quinone oxidoreductases and NADPH-/NADH dehydrogenases, catechol dioxygenase, homogentisate 1,2-dioxygenase, protocatechuate 3,4-dioxygenase, gentisate 1,2-dioxygenase, azobenzene reductase, naphthalene 1,2-dioxygenase, benzoate/toluate 1,2-dioxygenase, and anthranilate 1,2-dioxygenase and so on were found in the metagenome of the consortium BCGR-T. Finally, a new decolorization pathway of the thermophilic consortium BCGR-T was proposed. In addition, the phototoxicity of BCGR decreased after decolorization. Overall, the thermophilic consortium BCGR-T could be a promising candidate in the treatment of high concentration azo dye wastewater at high temperatures.

扩展阅读：

梁承月

环境科学与工程，讲师，硕士生导师

云南省“兴滇英才支持计划”青年人才

学术任职及兼职

担任Microbial Biotechnology、Water Reuse等国际期刊审稿人

主要招生专业：

1. 农业工程

2. 农业管理

3. 农业工程与信息技术

主要研究方向：

1. 有机污染水体/土壤的生物修复原理与技术

2. 有机废弃物资源的生物转化技术与原理

3. 功能微生物菌剂开发

学历及研究简历：

2008年9月-2012年6月，华中农业大学，本科生，生命科学与技术国家人才培养基地班

2011年9月-2014年6月，华中农业大学，硕士研究生，环境科学

2014年9月-2019年7月，清华大学，博士研究生，环境科学与工程

2020年8月-至今      ，云南师范大学，讲师

近三年代表论文和著作：

1. Chengyue Liang; Quanhui Ye; Yong Huang; Zuotao Zhang; Chongyang Wang; Yun Wang; Hui Wang; Distribution of the new functional marker gene (pahE) of aerobic polycyclic aromatic hydrocarbon (PAHs) degrading bacteria in different ecosystems. Science of the Total Environment, 2023, 865: 161233. (SCI I区，Top 期刊)

2. Chengyue Liang; Quanhui Ye; Yong Huang; Yun Wang; Zuotao Zhang; Hui Wang; Shifts of the new functional marker gene (pahE) of polycyclic aromatic hydrocarbons (PAHs) degrading bacterial population and its relationship with PAHs biodegradation, Journal of Hazardous Materials, 2022, 437(5), 129305. (SCI I区，Top 期刊)

3. Chengyue Liang; Yong Huang; Hui Wang; pahE, a functional marker gene for polycyclic aromatic hydrocarbon-degrading bacteria, Applied and Environmental Microbiology, 2019, 85(3): e02399-18. (SCI Ⅱ区，Top 期刊)

4. Chengyue Liang; Yong Huang; Yun Wang; Quanhui Ye; Zuotao Zhang; Hui Wang; Distribution of bacterial polycyclic aromatic hydrocarbon (PAH) ring-hydroxylating dioxygenases genes in oilfield soils and mangrove sediments explored by gene-targeted metagenomics, Applied Microbiology and Biotechnology, 2019, 103: 2427-2440. (SCI Ⅱ区，Top 期刊)

5. Quanhui Ye; Chengyue Liang; Xunwen Chen; Tingting Fang; Yun Wang; Hui Wang; Molecular characterization of methanogenic microbial communities for degrading various types of polycyclic aromatic hydrocarbon, Journal of Environmental Sciences, 2019, 86: 97-106. (SCI Ⅱ区)

6. Quanhui Ye; Chengyue Liang; Chongyang Wang; Yun Wang; Hui Wang; Characterization of a phenanthrene-degrading methanogenic community, Frontiers of Environmental Science and Engineering, 2018, 12(5): 4. (SCI Ⅱ区)

7. Lifei Zou; Changmei Wang; Xingling Zhao; Kai Wu; Chengyue Liang; Fang Yin; Bin Yang; Jing Liu; Hong Yang; Wudi Zhang; Enhanced anaerobic digestion of swine manure via a coupled microbial electrolysis cell, Bioresource Technology, 2021, 340: 125619. (SCI Ⅰ区，Top 期刊)

8. 罗志伟, 刘健峰, 王昌梅, 赵兴玲, 吴凯, 尹芳, 梁承月, 张无敌. 基于氨氮低吸附的折流式沉降滤过装置去除沼液中悬浮物研究. 农业现代化研究, 2022, 43(2): 360-368.（中文核心期刊）

9. 向锦茜, 张路聃, 梁承月*, 王昌梅, 尹芳, 吴凯, 杨斌, 赵兴玲, 张无敌*. 芘对剩余活性污泥厌氧消化的影响。云南师范大学学报（自然科学版）, 2022,42(06):1-4.

近五年获奖成果和荣誉称号：

1. 2018.11 International Academic Conference for Graduate Students of Nanjing Agricultural University, Oral Second Prize, Nanjing。

2. 2019.06“第八届地质微生物微生物学学术研讨会”获“徐怀恕优秀墙报奖”，青岛。

3. 2022.08第七届环境微生物利用与风险控制研讨会特邀报告，内蒙古呼和浩特市。

4. 2023.05第二届云南省大学生节能减排社会实践与科技竞赛，优秀指导教师奖。

近三年主持的国家级或百万以上其他项目：

1. 云南省科技厅，云南省基础研究专项（青年项目），2022/06-2025/05，在研，主持。

2. 云南省教育厅，科学研究基金项目，2022/03-2023/02，在研，主持。

3. 云南师范大学，博士科研启动项目（理科），2021/01-2025/12，在研，主持。

4. 国家自然科学基金委员会，面上项目，2018/01-2021/12，结题，参加。

近三年主持的教学教改及省级以上教学质量工程：

² 云南师范大学2022年课程思政建设项目（2022szkc23）

近三年主讲课程（研究生、本科生）：

生物质能工程，专业英语阅读，科技论文检索与写作，环境与健康

欢迎对研究方向感兴趣的研究生和优秀本科生进入实验室学习，联系邮箱：liangcy@ynnu.edu.cn。