HEIP1调控水稻减数分裂过程中的交叉形成

HEIP1 regulates crossover formation during meiosis in rice

在减数分裂过程中，双链断裂（double-strand breaks，DSBs）数量远远超过交叉（crossovers，COs）的最终数量。因此，鉴定哪些蛋白质参与确定DSBs修复为COs，对于理解交叉控制机制至关重要。

不同物种中，HEI10相关蛋白在交叉形成中起重要作用。本文通过酵母双杂交系统筛选HEI10互作蛋白，鉴定了水稻中的HEI10互作蛋白：HEI10 Interaction Protein 1 (HEIP1)……。

During meiosis, the number of double-strand breaks (DSBs) far exceeds the final number of crossovers (COs). Therefore, to identify proteins involved in determining which of these DSBs repaired into COs is critical in understanding the mechanism of CO control. Across species, HEI10-related proteins play important roles in CO formation. Here, through screening for HEI10-interacting proteins via a yeast two-hybrid system, we identify a CO protein HEI10 Interaction Protein 1 (HEIP1) in rice. HEIP1 colocalizes with HEI10 in a dynamic fashion along the meiotic chromosomes and specially localizes onto crossover sites from late pachytene to diplotene. Between these two proteins, HEI10 is required for the loading of HEIP1, but not vice versa. Moreover, mutations of the HEIP1 gene cause the severe reduction of chiasma frequency, whereas early homologous recombination processes are not disturbed and synapsis proceeds normally. HEIP1 interacts directly with ZIP4 and MSH5. In addition, the loading of HEIP1 depends on ZIP4, but not on MER3, MSH4, or MSH5. Together, our results suggest that HEIP1 may be a member of the ZMM group and acts as a key element regulating CO formation.

全文：

Baoxiang Qin_PNAS_2018.pdf