海洋大型藻类生物活性甘油糖脂的分离纯化及其食品防腐潜力

孙颖颖^1,2,3,4，张宇¹，茅陈炜¹，穆杨^1,5，李田乐¹，蒋秀婧¹，胡亚东⁶，潘明轩⁶，禹酉¹，王皓楠¹，韦欣^1*

(1. 江苏海洋大学海洋生物资源与环境重点实验室，连云港222005，江苏，中国；

2. 江苏海洋生物产业技术创新中心，连云港 222005，江苏，中国；3. 江苏省海洋资源开发研究所，连云港 222005，江苏，中国；4. 江苏海洋大学海洋遗传资源与育种重点实验室，连云港 222005，江苏，中国；5. 江苏中金马泰医药包装有限公司，连云港 222002，江苏，中国；6. 江苏沿海开发集团有限公司，南京 210095，中国)

摘要：大型藻类富含具有抗氧化和抗炎活性的生物活性甘油糖脂，是一类重要的海洋资源。该研究系统优化了掌状红皮藻（Palmaria palmata）、绳藻（Chorda filum）和条浒苔（Enteromorpha clathrata）中甘油糖脂的提取工艺。

通过单因素实验和响应面法，确定了最佳提取条件，三个物种的甘油糖脂得率分别达到69.96 mg/g、65.14 mg/g和85.73 mg/g。随后，对所得提取物的抗氧化及吸湿保湿活性进行了评价。

结果表明，甘油糖脂提取物对DPPH、羟基和ABTS自由基的清除能力呈现显著的浓度依赖性增强，但其整体效果仍低于维生素C。在吸湿和保湿性能方面，提取物在不同湿度条件下均优于透明质酸，但弱于甘油。进一步采用液-液萃取、薄层色谱和硅胶柱色谱纯化，获得了特定的甘油糖脂组分。此外，将所得甘油糖脂用于处理黑鱼（Channa argus）鱼片后，其理化指标较对照组有所改善，有效延缓了腐败进程。上述发现揭示了海洋大型藻类来源的甘油糖脂在食品防腐中的潜在应用价值，可为其在食品工业中的深入研究和实际应用奠定基础。

关键词：大型海藻；甘油糖脂；响应面设计；抗氧化活性；食品保鲜

DOI: 10.25165/j.ijabe.20261901.10113

引用信息: Sun Y Y, Zhang Y, Mao C W, Mu Y, Li T L, Jiang X J, et al. Bioactive glyceroglycolipids from marine macroalgae: Isolation, purification, and food preservation potential. Int J Agric & Biol Eng, 2026; 19(1): 270–282.

阅读全文链接：http://ijabe.net/article/doi/10.25165/j.ijabe.20261901.10113

Bioactive glyceroglycolipids from marine macroalgae: Isolation, purification, and food preservation potential

Yingying Sun^1,2,3,4, Yu Zhang¹, Chenwei Mao¹, Yang Mu^1,5, Tianle Li¹, Xiujing Jiang¹, Yadong Hu⁶, Mingxuan Pan⁶, You Yu¹, Haonan Wang¹, Xin Wei^1*

(1. Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China; 

2. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, Jiangsu, China; 

3. Jiangsu Institute of Marine Resources Development, Lianyungang 222005, Jiangsu, China; 

4. Jiangsu Key Laboratory of Marine Genetic Resources and Breeding, Jiangsu Ocean University, Lianyungang 222005, Jiangsu, China; 

5. Jiangsu Zhongjin Matai Medicinal Packaging Co., Ltd, Lianyungang 222002, Jiangsu, China;

 6. Jiangsu Coast Development Group Co., Ltd, Nanjing 210095, China)

Abstract: Macroalgae represent valuable marine resources, being rich in bioactive glyceroglycolipids with antioxidant and anti inflammatory properties. In this study, the extraction processes for glyceroglycolipids from Palmaria palmata, Chorda filum, and Enteromorpha clathrata were systematically optimized. Using single-factor and response surface methodologies, optimal extraction conditions were established, achieving yields of 69.96 mg/g, 65.14 mg/g, and 85.73 mg/g, respectively. The resultant extracts were subsequently evaluated for their antioxidant and hygroscopic-moisturizing activities. It was observed that the glyceroglycolipid extracts exhibited a significant, concentration-dependent increase in the scavenging of DPPH, hydroxyl, and ABTS radicals, although their overall efficacy remained lower than that of vitamin C. In terms of hygroscopic and moisturizing properties, the extracts performed better than hyaluronic acid but were inferior to glycerol across varying humidity conditions. Further purification employing liquid-liquid extraction, thin-layer chromatography, and silica gel column chromatography enabled the isolation of specific glyceroglycolipid fractions. Additionally, treatment of Channa argus fillets with these glyceroglycolipids resulted in improved physicochemical indices compared to the control group, effectively delaying spoilage. These findings underscore the potential of marine macroalgae-derived glyceroglycolipids in food preservation, providing a solid foundation for further research and application within the food industry.

Keywords: macroalgae, glyceroglycolipids, response surface design, anti-oxidant activity, food preservation

DOI: 10.25165/j.ijabe.20261901.10113

Citation: Sun Y Y, Zhang Y, Mao C W, Mu Y, Li T L, Jiang X J, et al. Bioactive glyceroglycolipids from marine macroalgae: Isolation, purification, and food preservation potential. Int J Agric & Biol Eng, 2026; 19(1): 270–282.

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