A reactive oxygen species burst causes haploid induction in maize

第一作者：Chenglin Jiang

第一单位：华中农业大学

通讯作者：Jianbing Yan

 Abstarct 

背景+问题：Haploid induction is an important tool in crop breeding. Phospholipase A1 (ZmPLA1) / NOT LIKE DAD (NLD)/ MATRILINEAL (MTL) is a key gene controlling haploid induction in maize; however, its molecular mechanism is unknown. 

单倍体诱导是作物育种中的一个重要技术。ZmPLA1/NLD/MTL是一个控制玉米单倍体诱导的关键基因；但是，有关其作用的分子机制还不清楚。

主要研究：To understand the regulatory network of zmpla1, we applied a comprehensive functional analysis at multiple omic levels, integrating transcriptomes, metabolomes, quantitative proteomes and protein modifications. 

为了理解zmpla1的调控网络，作者结合转录组、代谢组、定量蛋白组和蛋白修饰等多组学分析，对zmpla1进行了全面的功能分析。

结果1-ROS：Functional classes of significantly enriched or differentially abundant molecular entities were associated with the oxidative stress response, suggesting that a reactive oxygen species (ROS) burst plays a critical role in haploid induction. In support of this, we show that a simple chemical treatment of pollen with ROS reagents leads to haploid induction (HI). 

显著富集或差异富集的分子功能分类主要与氧化应激响应有关，表明活性氧物质（ROS）的爆发在单倍体诱导中起关键作用。作者发现用活性氧试剂对花粉进行简单的化学处理，即可导致单倍体诱导。

结果2-ZmPOD65：Moreover, a sperm-specific peroxidase (ZmPOD65) was identified as a new gene controlling HI. 

此外，一个精子特异性表达的过氧化物酶ZmPOD65被鉴定为控制单倍体诱导的关键基因。

结论：The mechanism of HI revealed in this study suggests the importance of ROS balance in maintaining normal reproduction, and provides a potential route to accelerate crop breeding.

本文所揭示的单倍体诱导机制表明了ROS平衡在维持正常生殖中的重要性，并且为加速作物育种提供了一条潜在途径。

** 严建兵 **

doi: https://doi.org/10.1016/j.molp.2022.04.001

Journal: Molecular Plant

Published date:  April 04, 2022