The microRNA476a‐RFL module regulates adventitious root formation through a mitochondria‐dependent pathway in Populus

第一作者：Changzheng Xu

第一单位：西南大学

通讯作者：Keming Luo

 Abstract 

背景回顾：For woody plants, clonal propagation efficiency is largely determined by adventitious root(AR) formation at the bases of stem cuttings.

提出问题：However, our understanding of the molecular mechanisms contributing to AR morphogenesis in trees remains limited, despite the importance of vegetative propagation, currently the most common practice for tree breeding and commercialization.

主要发现：Here, we identified Populus‐specific miR476a as a regulator of wound‐induced adventitious rooting that acts by orchestrating mitochondrial homeostasis.

结果1-MiR476a表达pattern与下游基因：MiR476a exhibited inducible expression during AR formation and directly targeted several Restorer of Fertility like (RFL) genes encoding mitochondrion‐localized pentatricopeptide repeat proteins.

结果2-miR476a‐RFL调控模块：Genetic modification of miR476a‐RFL expression revealed that miR476a/RFL‐mediated dynamic regulation of mitochondrial homeostasis influences AR formation in poplar.

结果3-生长素信号转导：Mitochondrial perturbation via exogenous application of a chemical inhibitor indicated that miR476a/RFL‐directed AR formation depends on mitochondrial regulation that acts via auxin signaling.

结论：Our results thus establish a miRNA‐directed mitochondrion–auxin signaling cascade required for AR development, providing insights into the role of mitochondrial regulation in the developmental plasticity of plants.

doi: https://doi.org/10.1111/nph.17252

Journal: New Phytologist

Published date: Feb 03, 2021