主要研究：We induced TW in the stems of California poplar (Populus trichocarpa, Nisqually-1) and identified two significantly repressed transcription factor (TF) genes: class B3 Heat-Shock Transcription Factor (HSFB3-1) and MYB092.

结果1-转录调控网络构建：Transcriptomic analysis and chromatin immunoprecipitation (ChIP) was used to identify direct TF–DNA interactions in P. trichocarpa xylem protoplasts overexpressing the TFs. This analysis established a transcriptional regulatory network in which PtrHSFB3-1 and PtrMYB092 directly activate 8 and 11 monolignol genes, respectively.

结果2-试验验证：The TF–DNA interactions were verified for their specificity and transactivator roles in 35 independent CRISPR-based biallelic mutants and overexpression transgenic lines of PtrHSFB3-1 and PtrMYB092 in P. trichocarpa.

结果3-敲除和过表达表型：The gene-edited trees (mimicking the repressed PtrHSFB3-1 and PtrMYB092 under tension stress) have stem wood composition resembling that of TW during normal growth and under tension stress (i.e., low lignin and high cellulose), whereas the overexpressors showed an opposite effect (high lignin and low cellulose).

结果4：Individual overexpression of the TFs impeded lignin reduction under tension stress and restored high levels of lignin biosynthesis in the TW.

结论：This study offers biological insights to further uncover how metabolism, growth, and stress adaptation are coordinately regulated in trees.