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第一作者:Xu Wang
第一单位:德国霍恩海姆大学
通讯作者:Jung-Youn Lee
Abstract
大背景:Numerous cell surface receptors and receptor-like proteins (RLPs) undergo activation or deactivation via a transmembrane domain (TMD).
小背景:A subset of plant RLPs distinctively localizes to the plasma membrane-lined pores called plasmodesmata. Those RLPs include the Arabidopsis thaliana Plasmodesmata-located protein (PDLP) 5, which is well known for its vital function regulating plasmodesmal gating and molecular movement between cells.
主要发现:In this study, we report that the TMD, although not a determining factor for the plasmodesmal targeting, serves essential roles for the PDLP5 function.
结果:In addition to its role for membrane anchoring, the TMD mediates PDLP5 self-interaction and carries an evolutionarily conserved motif that is essential for PDLP5 to regulate cell-to-cell movement. Computational modeling-based analyses suggest that PDLP TMDs have high propensities to dimerize.
讨论:We discuss how a specific mode(s) of TMD dimerization might serve as a common mechanism for PDLP5 and other PDLP members to regulate cell-to-cell movement.
摘 要
许多细胞表面受体和类受体蛋白RLPs会通过跨膜结构域TAD经历激活或失活。有一小类植物的RLPs会独特地定位于连接质膜的孔道,即胞间连丝上。这些RLPs包括拟南芥的胞间连丝定位蛋白PDLP5,该蛋白主要参与胞间连丝通道门控以及细胞间的分子移动。本文中,作者发现虽然TMD不是PDLP5蛋白靶向胞间连丝的决定性因子,但是对于PDLP5蛋白发挥功能具有不可或缺的作用。PDLP5蛋白中的TMD除了发挥膜锚定的作用,其还介导了PDLP5蛋白的自相互作用,并且携带一个演化上十分保守的基序,该基序对于PDLP5调控细胞间移动的功能至关重要。基于计算模型的分析显示PDLP蛋白的TMDs很容易发生二聚化。作者讨论了TMD二聚化的特异模式可能是一个通用的模式,作用于PDLP5以及其它PDLP蛋白调控细胞-细胞间的移动。
通讯作者
**Jung-Youn Lee**
个人简介:
佛罗里达大学,硕士;
佛罗里达大学,博士;
加州大学戴维斯分校,博士后。。
研究方向:植物细胞间通讯的分子机制。
doi: 10.1038/s42003-020-1007-0
Journal: Communications Biology
Published date: June 05, 2020
p.s. 通讯往期精彩链接:
p.s. 往期植物胞间连丝相关研究链接:
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