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First author: Maritza van Dop; Affiliations: Wageningen University (瓦赫宁根大学): Wageningen, the Netherlands
Corresponding author: Dolf Weijers
Cell polarity is fundamental for tissue morphogenesis in multicellular organisms. Plants and animals evolved multicellularity independently, and it is unknown whether their polarity systems are derived from a single-celled ancestor. Planar polarity in animals is conferred by Wnt signaling, an ancient signaling pathway transduced by Dishevelled, which assembles signalosomes by dynamic head-to-tail DIX domain polymerization. In contrast, polarity-determining pathways in plants are elusive. We recently discovered Arabidopsis SOSEKI proteins, which exhibit polar localization throughout development. Here, we identify SOSEKI as ancient polar proteins across land plants. Concentration-dependent polymerization via a bona fide DIX domain allows these to recruit ANGUSTIFOLIA to polar sites, similar to the polymerization-dependent recruitment of signaling effectors by Dishevelled. Cross-kingdom domain swaps reveal functional equivalence of animal and plant DIX domains. We trace DIX domains to unicellular eukaryotes and thus show that DIX-dependent polymerization is an ancient mechanism conserved between kingdoms and central to polarity proteins.
细胞极性是多细胞生物组织形态建成的基础。植物和动物独立演化出了多细胞特性,但对于其极性系统是否来源于同一个祖先还不清楚。动物中的平面细胞极性是由Wnt信号赋予的,该信号是由Dishevelled蛋白转导的古老信号通路,以动态头尾相连的方式聚合DIX结构域组成信号小体。相反,植物中决定极性的通路还是未知的。作者最近的研究报道了拟南芥中的SOSEKI蛋白在发育过程中存在极性定位(https://doi.org/10.1038/s41477-019-0363-6)。本文中,作者发现SOSEKI作为一个古老的极性蛋白在所有的陆地植物中均存在。由DIX结构域决定的浓度依赖性聚合使得这些蛋白能够招募ANGUSTIFOLIA到极性位点,这与Dishevelled蛋白依赖于聚合招募信号转导效应子十分相似。通过彼此交换动、植物中的DIX结构域,发现这两个不同界生物的DIX结构域功能具有等效性。作者追踪DIX结构域的起源直至单细胞的真核生物,说明DIX结构域依赖型的聚合是一个古老的机制,在不同界多细胞生物之间十分保守,同时是极性蛋白的核心部分。
通讯:Dolf Weijers (https://f1000.com/prime/thefaculty/member/1058938112246895)
个人简介:1997年,荷兰恩斯赫德,生物化学学士;2002年,荷兰莱顿大学,发育遗传学博士;2002-2006年,德国图宾根大学,Gerd Jürgens实验室博士后。
研究方向:以拟南芥早期胚胎会材料研究细胞如何获取其特性。
doi: https://doi.org/10.1016/j.cell.2020.01.011
Journal: Cell
Published date: January 30, 2020
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