A reference-grade wild soybean genome

First author: Min Xie; Affiliations: The Chinese University of Hong Kong (香港中文大学): Hong Kong, China

Corresponding author: Hon-Ming Lam

Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Here we report a reference-grade genome of wild soybean accession W05, with a final assembled genome size of 1013.2 Mb and a contig N50 of 3.3 Mb. The analytical power of the W05 genome is demonstrated by several examples. First, we identify an inversion at the locus determining seed coat color during domestication. Second, a translocation event between chromosomes 11 and 13 of some genotypes is shown to interfere with the assignment of QTLs. Third, we find a region containing copy number variations of the Kunitz trypsin inhibitor (KTI) genes. Such findings illustrate the power of this assembly in the analysis of large structural variations in soybean germplasm collections. The wild soybean genome assembly has wide applications in comparative genomic and evolutionary studies, as well as in crop breeding and improvement programs.

作物的遗传改良效率依赖于我们对不同种质资源所包含的遗传信息的了解程度。本文报道了野生大豆W05的参考基因组序列，基因组组装大小为1013.2Mb，contig N50大小为3.3 Mb。作者鉴定了一个决定大豆驯化过程中种皮颜色的位点倒位。某些基因型11号染色体和13号染色体之间的易位能够干扰数量性状位点的定位。作者还在基因组上鉴定到了一个含有KTI基因拷贝数变异的区域。这些发现说明了本文所报道的野生大豆的组装有助于大豆种子资源的结构变异分析。本文所报道的野生大豆基因组将有利于比较基因组和演化方向的研究，同样能够帮助作物育种和遗传改良项目。

通讯：Hon-Ming Lam (http://rice.njau.edu.cn/info/1012/1049.htm)

个人简介：1985年，香港中文大学，学士；1987年，香港中文大学，硕士；1992年，西北大学，博士。

研究方向：智能可持续型农业；植物和农业生物技术；作物与环境相互作用的基因组学研究。

doi: https://doi.org/10.1038/s41467-019-09142-9

Journal: Nature Communications

First Published: March 14, 2019