DELAYED HEADING DATE1 interacts with OsHAP5C/D, delays flowering time and enhances yield in rice

First author: Huan Zhang; Affiliations: Nanjing Agricultural University (南京农业大学): Nanjing, China

Corresponding author: Jianmin Wan

Heading date (抽穗期) is an important agronomic trait affecting crop yield. The GRAS protein family is a plant‐specific super family extensively involved in plant growth and signal transduction. However, GRAS proteins are rarely reported have a role in regulating rice heading date. Here, we report a GRAS protein DHD1 (Delayed Heading Date1) delays heading and enhances yield in rice. Biochemical assays showed DHD1 physically interacts with OsHAP5C/D both in vitro and in vivo. DHD1 and OsHAP5C/D located in the nucleus and showed that rhythmic expression (节律性表达). Both DHD1 and OsHAP5C/D affect heading date by regulating expression of Ehd1. We propose that DHD1 interacts with OsHAP5C/D to delay heading date by inhibiting expression of Ehd1.

抽穗期是一个非常重要的农艺性状，对于作物产量有着重要的影响。GRAS蛋白是一类植物特异的超家族蛋白，其广泛作用于植物的生长和信号转导。然而，关于GRAS蛋白在水稻抽穗期的作用报道的很少。本文的研究显示水稻GRAS蛋白DHD1延迟了抽穗期并增加了水稻产量。生物化学试验显示无论在体内或是体外条件下，DHD1都能够物理上与OsHAP5C/D进行互作。DHD1和OsHAP5C/D蛋白均定位于核中，并且具有节律性的表达模式。DHD1和OsHAP5C/D蛋白均通过调控Ehd1基因的表达影响水稻抽穗期。作者认为DHD1通过与OsHAP5C/D互作以抑制Ehd1基因的表达，最终延迟水稻抽穗期。

通讯：万建民 (http://rice.njau.edu.cn/info/1012/1049.htm)

个人简介：1982年，南京农业大学，农学学士；1985年，南京农业大学，作物遗传育种硕士；1995年，日本京都大学，农学博士。

研究方向：水稻优异基因挖掘和分子育种研究。

doi: https://doi.org/10.1111/pbi.12996

Journal: Plant Biotechnology Journal

Published date: 14 August, 2018

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