Visualization of nitroxyl (HNO) in vivo via a lysosome-targetable near-infrared fluorescent probe

We have presented a near-infraredfluorescent probe Lyso-JN for the detection ofnitroxyl (HNO) in cells and in vivo. Lyso-JN is comprised of three moieties: an Aza-BODIPY fluorophore, a HNO-response modulatordiphenylphosphino-benzoyl, and a lysosomal locator alkylmorpholine. The detection mechanism is based onaza-ylide intramolecular ester aminolysis reactionupon reaction with HNO. The probe holds ability to capture lysosomal HNO in RAW 264.7 cells，and also successfully employed to visualize HNO in mice.

该工作设计了一种基于BODPY的近红外荧光探针用于细胞溶酶体内HNO的检测。作为一氧化氮的单电子还原产物，亚硝酰氢 (HNO) 的生理作用与NO迥异。HNO可增加正常及心力衰竭时心肌的收缩力，同时改善心肌舒张功能。因此，开发用于检测HNO的荧光探针，为进一步探究HNO的生理学意义和药物价值提供了有力的手段。通过实验，我们证实Lyso-JN不仅可以穿透更深的组织和有效地避免背景干扰，而且还具有高选择性、良好的灵敏度和低细胞毒性。这种小分子荧光成像探针能够成功定位于细胞溶酶体，并且很好的实现了RAW264.7 细胞和BALB/c小鼠成像。

Xiaotong Jing,‡ Fabiao Yu‡ and Lingxin Chen* Chem. Commun., 2014, 50, 14253