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云师大化工学院张旭锋副教授在《INT J BIOL MACROMOL》上发表最新研究成果

已有 280 次阅读 2024-12-30 22:04 |个人分类:云师大研究|系统分类:论文交流

2024年12月21日，Elsevier旗下top期刊《International Journal of Biological Macromolecules》在线发表了云南师范大学化学化工学院张旭锋副教授最新研究成果《

Purification and structural characterization of a neutral polysaccharide from Boletus auripes using self-made quaternary chitosan cryogel》。云南师范大学化学化工学学院为第一作者单位，云南师范大学化学化工学学院张旭锋副教授、武汉大学化学与分子科学学院Xiaojuan Xu和昆明植物研究所 Liang Zhang为共同通讯作者。

Abstract

The purification of polysaccharides is an essential preliminary step in determining their chemical structure, although it presents significant challenges. In this research, a macro-porous monolith of quaternary chitosan cryogel was synthesized for the purification of a neutral polysaccharide from Boletus auripes. A homogeneous neutral polysaccharide (BAP-1a1) with a weight-average molecular weight of 4.13 × 105 Da and a polydispersity index of 1.28 was successfully isolated. The structure of BAP-1a1 was elucidated through a comprehensive characterization utilizing size exclusion chromatography (SEC) combined with laser light scattering (LLS), infrared spectroscopy, monosaccharide composition analysis, methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy. The results revealed that the BAP-1a1 was characterized as a glucan with a backbone structure consisting of 1,4-α-D-Glcp and 1,3-β-D-Glcp glycosidic linkages in a molar ratio of 2:1. Additionally, a minority of branched chains of 1-α-D-Glcp are attached to 1,3-β-D-Glcp residues at the C6 position. In vitro antioxidant activity assays demonstrated that BAP-1a1 exhibits a dose-dependent scavenging effect on ABTS and DPPH radicals with EC50 values of 0.58 and 1.04 mg/mL, respectively. These findings indicated that Boletus auripes possesses the potential to be utilized as a natural agent in antioxidant functional foods.