Hepcidin-25是一种由25个氨基酸组成的肽类激素，在其肽链中存在着一条金属结合基序，并在脊椎动物铁代谢调控中发挥核心作用¹。作为铁稳态的"主调控因子"，它通过抑制肠道铁吸收和巨噬细胞铁释放来调控循环铁的水平²。Hepcidin-25常常作为疾病模型中的分析指标或者生物标志分子。例如在健康状态下，大鼠血浆中Hepcidin-25的平均浓度为63.0 ng/mL，而在脂多糖刺激后显著升高至218.2 ng/mL，表明Hepcidin-25与炎症反应高度相关³。在心血管领域，研究发现Hepcidin-25与动脉僵硬度存在关联⁴。Hepcidin-25还可调节铁代谢，Hepcidin-25与其受体铁输出蛋白结合后，可抑制肠道铁吸收以及肝细胞和巨噬细胞的铁外排。检测Hepcidin-25可以预测机体的铁代谢状态，并有助于研究贫血和某些以贫血为特征的动物疾病模型，例如慢性肾脏病（CKD）、糖尿病相关的慢性肾病、炎症性肠病血色病等动物模型⁵。Hepcidin-25的升高还可用于监测肿瘤的发生发展，例如Hepcidin-25与肝细胞癌的肾脏转移有关⁶，并且Hepcidin-25还可作为非侵入性乳腺癌预测工具。目前针对Hepcidin-25的检测手段包括LC-MS/MS法、ELISA检测试剂盒等技术。Hepcidin-25还具有其它的生物学应用例如抗菌，有研究表明Hepcidin-25的水平在细菌感染后会明显上调，表明其与机体的主动防御有关。而在体外实验中，Hepcidin-25及其衍生物Hepcidin-20对多种病原微生物表现出抑制活性⁷。

Hepcidin-25和Hepcidin-20对多药耐药性细菌的抑制浓度⁷

参考文献及鸣谢

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