高效便捷植物遗传转化新方法：传统植物转基因方法需通过组织培养等再生程序才能获得转基因植株，对难再生作物的转基因植物更加困难。发展高效、安全新型遗传转化方法是基因工程、分子生物学和遗传育种等领域的研究热点之一。

近日，Nature Plants发表了一种高通量、操作便捷的植物遗传转化新方法（Pollen magnetofection for genetic modification with magnetic nanoparticles as gene carriers），本文研发了基于磁性纳米颗粒基因载体的花粉磁转化植物遗传修饰新方法。该研究利用磁性纳米颗粒Fe3O4作为载体，在外加磁场介导下将外源基因输送至花粉内部，通过人工授粉过程直接获得转化种子，然后再经过选育获得稳定遗传的转基因后代。该方法将纳米磁转化和花粉介导法相结合，克服了传统转基因方法组织再生培养和寄主适应性等方面的瓶颈问题，对于加速转基因生物新品种培育具有重要意义（资料引自中国农业科学院新闻网）。

Abstract: Genetic modification plays a vital role inbreeding new crops with excellent traits. Almost all the current genetic modification methods require regeneration from tissue culture, involving complicated, long and laborious processes. In particular, many crop species such as cotton are difficult to regenerate. Here, we report a novel transformation platform technology, pollen magnetofection, to directly produce transgenic seeds without regeneration. In this system, exogenous DNA loaded with magnetic nanoparticles was delivered into pollen in the presence of amagnetic field. Through pollination with magnetofected pollen, transgenic plants were successfully generated from transformed seeds. Exogenous DNA was successfully integrated into the genome, effectively expressed and stably inherited in the offspring. Our system is culture-free and genotype independent. In addition, it is simple, fast and capable of multi-gene transformation. We envision that pollen magnetofection can transform almost allcrops, greatly facilitating breeding processes of new varieties of transgenic crops.