CALCIUM-DEPENDENT PROTEIN KINASE38 regulates flowering time and common cutworm resistance in soybean

第一作者：Xiao Li

第一单位：南京农业大学

第一通讯：Hui Wang

 Abstarct 

背景回顾：Photoperiod-sensitive plants such as soybean (Glycine max) often face threats from herbivorous insects throughout their whole growth period and especially during flowering; however, little is known about the relationship between plant flowering and insect resistance. 

大豆等对光周期敏感的植物，在其整个生长期尤其是开花期，经常会面临草食昆虫的威胁；然而，目前对于植物开花与抗虫性之间的关联知之甚少。

主要研究：Here, we used gene editing, multiple omics, genetic diversity and evolutionary analyses to confirm that the calcium-dependent protein kinase GmCDPK38 plays a dual role in coordinating flowering time regulation and insect resistance of soybean. 

本文中，作者利用基因编辑、多组学、遗传多样性以及演化分析表明，钙依赖性蛋白激酶GmCDPK38在协调大豆开花时间调控和抗虫性方面起着双重作用。

结果：Haplotype 2 (Hap2)-containing soybeans flowered later and were more resistant to the common cutworm (Spodoptera litura Fabricius) than those of Hap3. gmcdpk38 mutants with Hap3 knocked out exhibited similar flowering and resistance phenotypes as Hap2. Knocking out GmCDPK38 altered numerous flowering- and resistance-related phosphorylated proteins, genes, and metabolites. For example, the S-adenosylmethionine synthase GmSAMS1 was posttranslationally upregulated in the gmcdpk38 mutants. GmCDPK38 has abundant genetic diversity in wild soybeans and was likely selected during soybean domestication. We found that Hap2 was mostly distributed at low latitudes and had a higher frequency in cultivars than in wild soybeans, while Hap3 was widely selected at high latitudes. 

含有单倍型Hap2的大豆开花较晚，对斜纹夜蛾的抗性高于含有单倍型Hap3的大豆。gmcdpk38突变体的Hap3缺失，表现出与Hap2类似的开花习性和抗虫性表型。敲除GmCDPK38基因改变了许多与开花和抗性相关的磷酸化蛋白、基因和代谢物。例如，S-腺苷蛋氨酸合酶GmSAMS1在gmcdpk38突变体中受到翻译后的上调。GmCDPK38在野生大豆中具有丰富的遗传多样性，可能在大豆的驯化过程中受到了选择。作者还发现，含有Hap2的大豆主要分布在低纬度地区，在栽培种中出现的频率要高于野生大豆，而Hap3则是在高纬度地区被广泛选择。

结论：Overall, our results elucidated that the two distinct traits (flowering time and insect resistance) are mediated by GmCDPK38.

总的来说，本文的研究结果阐明了开花时间和抗虫性这两个完全不同的性状是由同一个基因，即GmCDPK38来介导的。

 王慧 

doi: https://doi.org/10.1093/plphys/kiac260

Journal: Plant Physiology

Published date: May 31, 2022

CITE

​Xiao Li, Dezhou Hu, Linyan Cai, Huiqi Wang, Xinyu Liu, Haiping Du, Zhongyi Yang, Huairen Zhang, Zhenbin Hu, Fang Huang, Guizhen Kan, Fanjiang Kong, Baohui Liu, Deyue Yu, Hui Wang. CALCIUM-DEPENDENT PROTEIN KINASE38 regulates flowering time and common cutworm resistance in soybean. Plant Physiology, 2022. DOI: https://doi.org/10.1093/plphys/kiac260