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第一作者:Bin Ma
第一单位:上海理工大学
通讯作者:Zuhua He
Abstract
背景回顾:Phosphate (Pi) is essential to plant growth and crop yield.
提出问题:However, it remains unknown how Pi homeostasis is maintained during cereal grain filling.
主要发现:Here, we identified a rice grain-filling-controlling PHO1-type Pi transporter, OsPHO1;2, through map-based cloning.
结果1-表达模式+酶活:Pi efflux activity and its localization to the plasma membrane of seed tissues implicated a specific role for OsPHO1;2 in Pi reallocation during grain filling.
结果2-功能鉴定:Indeed, Pi over-accumulated in developing seeds of the Ospho1;2 mutant, which inhibited the activity of ADP-glucose pyrophosphorylase (AGPase), important for starch synthesis, and the grain-filling defect was alleviated by overexpression of AGPase in Ospho1;2-mutant plants.
结果3-功能保守:A conserved function was recognized for the maize transporter ZmPHO1;2.
结果4-应用潜力:Importantly, ectopic overexpression of OsPHO1;2 enhanced grain yield, especially under low-Pi conditions.
结论:Collectively, we discovered a mechanism underlying Pi transport, grain filling and P-use efficiency, providing an efficient strategy for improving grain yield with minimal P-fertilizer input in cereals.
摘 要
物生长和作物产量是必须的。但是,谷类作物在籽粒灌浆时期是如何维持磷的动态平衡的还不清楚。本文中,作者基于图位克隆,在水稻中鉴定到了一个控制籽粒灌浆PHO1型磷转运蛋白OsPHO1;2。OsPHO1;2在种子组织中表达,定位于质膜,且具有磷外流活性,表明该蛋白在水稻籽粒灌浆时期的磷再分配过程中发挥重要作用。确实,作者发现Ospho1;2突变体的正在发育的种子会过量积累磷,该突变会抑制淀粉合成相关酶AGPase的活性,并且作者通过在Ospho1;2突变体中过表达AGPase能够减缓籽粒灌浆的缺陷。另外,作者发现玉米中的ZmPHO1;2具有与水稻OsPHO1;2保守的功能。重要的是,异位表达OsPHO1;2能够增强籽粒产量,尤其是在低磷条件下。综上,作者揭示了一个作用于磷转运、籽粒灌浆以及磷利用效率的分子机制,并为未来培育在低磷条件下高产的谷类作物这一育种目标提供了有效策略。
通讯作者
** 何祖华 **
个人简介:
1993-1996年,浙江大学,博士;
1997-1998年,美国加州Salk研究所,博后;
1999-2000年,加州大学Davis分校,博后。
研究方向:植物抗病信号转导、功能基因和信号途径互作。
doi: https://doi.org/10.1038/s41588-021-00855-6
Journal: Nature Genetics
First Published: Apr 29, 2021
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