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第一作者:Matthew T Parker
第一单位:英国邓迪大学
通讯作者:Gordon Grant Simpson
Abstract
背景回顾:Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. 研究意义:However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. 主要发现:Here we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. 结果:Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. 结论:Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants. 摘 要
参与疾病抗性的基因是基因组演化最快、多态性最高的组份。植物基因组中存在大量的NLR基因,这些基因对于植物的疾病抗性至关重要。但是,NLRs会诱导植物的自发免疫,干扰有益微生物群或降低适应性。因此,理解NLR是如何被控制的十分重要。本文中,作者发现RNA结合蛋白FPA介导了NLR转录本广泛的过早剪切和聚腺苷酸化,从而控制其功能表达,并影响植株免疫。利用长读长的Nanopore直接RNA测序,作者解析了NLR转录本加工的复杂性和基因注释。本文的结果揭示了一个NLR控制的共转路层级,为NLR基因在植物免疫响应中的调控和演化动态提供了新的视野。
通讯作者
** Gordon Grant Simpson **
研究方向:RNA和RNA加工在控制植物发育中的作用。
doi: https://doi.org/10.7554/eLife.65537
Journal: eLife
Published date: Apr 27, 2021
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