Sucrose promotes stem branching through cytokinin

第一作者：Bolaji Babajide Salam

第一单位：以色列农业研究中心

通讯作者：Dani Eshel

 Abstract 

背景回顾：Shoot branching is an important aspect of plant architecture because it substantially affects plant biology and agricultural performance. Sugars play an important role in the induction of shoot branching in several species, including potato (Solanum tuberosum L.).

提出问题：However, the mechanism by which sugars affect shoot branching remains mostly unknown.

主要研究：In the present study, we addressed this question using sugar-mediated induction of bud outgrowth in potato stems under etiolated conditions.

结果1-CK & Vlnv：Our results indicate that sucrose feeding to detached stems promotes the accumulation of cytokinin (CK), as well as the expression of vacuolar invertase (VInv), an enzyme that contributes to sugar sink strength.

结果2-CK抑制剂与CK处理：These effects of sucrose were suppressed by CK synthesis and perception inhibitors, while CK supplied to detached stems induced bud outgrowth and VInv activity in the absence of sucrose.

结果3-vinv突变体：CK-induced bud outgrowth was suppressed in vinv mutants, which we generated by genome editing.

结论：Altogether, our results identify a branching-promoting module, and suggest that sugar-induced lateral bud outgrowth is in part promoted by the induction of CK-mediated VInv activity.

 摘 要 

茎分枝是植物结构的重要性状，其极大地影响了植物的生物学特性以及农艺价值。在土豆等少数物种中，蔗糖在茎分枝的诱导过程中发挥着重要作用。但是，蔗糖具体如何作用于茎分枝的诱导还不清楚。本文中，作者利用黄化的马铃薯苗茎，采用蔗糖介导的芽向外生长来研究这个问题。作者的研究显示，对离体的马铃薯茎进行蔗糖供应能够促进细胞分裂素的积累，同时还会促进一个能够增强蔗糖“库”强度的液泡转化酶VInv的表达。通过CK合成和感知抑制能够抑制蔗糖的这些效应，而对离体的马铃薯茎进行细胞分裂素处理，在缺失蔗糖的情况下仍旧能够诱导芽向外生长以及VInv活性。作者通过基因组编辑产生的vinv突变体中，细胞分裂素诱导的芽向外生长被抑制。综上，本文的研究鉴定了一个分枝促进模块，揭示了蔗糖诱导的侧芽生长部分是通过细胞分裂素介导的VInv活性来促进的。

 通讯作者 

**Dani Eshel**

doi: https://doi.org/10.1093/plphys/kiab003

Journal: Plant Physiology

Published date: Jan 13, 2021