Single-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery

第一作者：Xiaosa Xu

第一单位：冷泉港实验室

通讯作者：David Jackson

 Abstract 

背景回顾：Crop productivity depends on activity of meristems that produce optimized plant architectures, including that of the maize ear. A comprehensive understanding of development requires insight into the full diversity of cell types and developmental domains and the gene networks required to specify them.

存在问题：Until now, these were identified primarily by morphology and insights from classical genetics, which are limited by genetic redundancy and pleiotropy.

主要研究：Here, we investigated the transcriptional profiles of 12,525 single cells from developing maize ears.

结果1-单细胞转录图谱：The resulting developmental atlas provides a single-cell RNA sequencing (scRNA-seq) map of an inflorescence.

结果2-数据验证与利用：We validated our results by mRNA in situ hybridization and by fluorescence-activated cell sorting (FACS) RNA-seq and we show how these data may facilitate genetic studies by predicting genetic redundancy, integrating transcriptional networks, and identifying candidate genes associated with crop yield traits.

 摘 要 

doi: https://doi.org/10.1016/j.devcel.2020.12.015

Journal: Current Biology

Published date: Jan 04, 2021