Embryonic development of the concave-eared torrent frog

with its significance on taxonomy

XIONG Rong-Chuan1,2, JIANG Jian-Ping1,* , FEI Liang1, WANG Bin1,2, YE Chang-Yuan1

(1. Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu  610041, China;

2. Graduate University of the Chinese Academy of Sciences, Beijing  100049, China)

Abstract: We investigated the early embryonic and larval development of the concave-eared torrent frogs, Odorrana tormota (Amphibia, Anura, Ranidae). Embryos were derived from artificial fertilization of frogs’ eggs, and the staging of development was based on morphological and physiological characteristics. Two major periods of development were designated: i) early embryonic period, from fertilization to operculum completion stage, lasted for 324 h at water temperature (WT) 18 −23℃; ii) larval period, from operculum completion stage to tail absorbed stage, took 1207 h at WT 20 − 24℃. Tadpoles of the concave-eared torrent frogs showed no evidence of abdominal sucker. Absence of this key characteristic supports the view from molecular systematics that concave-eared torrent frog does not belong to the genus Amolops. Two cleavage patterns were observed in embryos at 8-cell and 16-cell stages, with Pattern I - 2 (latitudinal cleavage at the 8-cell stage, and meridional cleavage at the 16-cell stage with two perpendicular meridional furrows) being the predominant pattern and only 1.5% belonging to Pattern II (meridional cleavage at the 8-cell stage and latitudinal cleavage at the 16-cell stage). The factors affecting cleavage and hatching ratios, developmental speed, and ecological adaptation were discussed.(熊荣川 et al., 2010)

Key words: Odorrana tormota; Embryonic development; Artificial fertilization; Abdominal sucker; Embryonic cleavage

凹耳臭蛙胚胎发育及其分类学意义

熊荣川1, 2，江建平1，*，费  梁1，王  斌1, 2，叶昌媛1

（1. 中国科学院成都生物研究所，四川 成都  610041; 2. 中国科学院研究生院，北京  100049）

摘要：通过人工受精的方法获得的凹耳臭蛙（Odorrana tormota）的早期胚胎及胚后幼体的发育过程，根据胚胎发育过程中的形态及生理特征变化规律进行分期。把凹耳臭蛙的发育过程分成两个阶段：1）早期胚胎发育阶段，即从蛙卵受精到鳃盖完成期，在18～23℃水温下，凹耳臭蛙早期胚胎发育阶段历时324 h；2）蝌蚪发育阶段，即从鳃盖完成期结束到尾部被完全吸收，本阶段在20～24℃水温条件下历时1 207 h。凹耳臭蛙蝌蚪未发现腹吸盘特征，从形态特征上支持了分子系统分类学将之从湍蛙属划出的观点。实验中发现，多数胚胎在8细胞期为纬裂，16细胞期为经裂，同时有小部分胚胎（1.5%）在8细胞期为经裂，16细胞期为纬裂。该文进一步讨论了影响卵裂率、孵化率、发育速度，以及生态适应的因素。

关键词：凹耳臭蛙；胚胎发育；人工受精；腹吸盘；卵裂

 

附件下载

 凹耳臭蛙胚胎发育及其分类学意义(英文).pdf

 

参考文献：

熊荣川, 江建平, 梁 费, 斌 王,叶昌媛 (2010). "凹耳臭蛙胚胎发育及其分类学意义(英文)." 动物学研究 31(5): 490-498.

 

 

 

转载本文请联系原作者获取授权，同时请注明本文来自熊荣川科学网博客。
链接地址：https://blog.sciencenet.cn/blog-508298-407346.html

上一篇：能区别邻居和陌生者的凹耳臭蛙
下一篇：岛屿种群遗传多样性是否低于大陆种群