PDF 文档的使用范围很广，但有的时候需要提取 PDF 文档却茫然无所适从。Adobe Acrobat Pro 是一个专业处理 PDF 文档的工具，可以方便地实现这一功能，但因其较高的价格，普及率并不高。许多在线工具也可以做到，只是有的版式混乱，有的网速较慢，不太方便。其实仅仅使用很常见的 Chrome 浏览器就可以实现这一目的。非常简 ...
MareyMap 是一个基于 R 语言的有图形界面的用于估计基因组重组率的软件（ Rezvoy et al, 2007 ）。具体安装环境为，操作系统版本 OS X Yosemite (10.10.4)；R 语言版本: version 3.2.2；MareyMap 软件版本 version 1.3.1。 首先，从 CRAN （The Comprehensive R Archive Network）网站上下载 MareyMap 最新版 ...
The command samtools fastq will directly convert a bam file to fastq files. Combining with the filter parameter, we could output unmapped read pairs by -f 12 . 0x0004 in the flag field of the SAM format means “the query sequence itself is unmapped” and 0x0008 & ...
Recently, I found that overlapping varaints existed in the results that called by GATK HaplotypeCaller (version 4.0.1.2). I noticed this because I want to build consensus sequences using bcftools consensus , which produced warnings like these: ThesiteChr01:597519 ...
Generally, for a program that support multi-threading, the elapsed time will reduce with the increasing number of used CPUs. However, I found a strang case that picard MarkDuplicates will run slower with more CPUs. When I run picard MarkDuplicates in a node with 160 CPUs, it ...
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