博文
Genotyping based on NGS
||
Genotyping based on NGS
NGS(next generation sequencing) is very useful for genotyping. In recently or in the future, genotyping based on NGS will become very popular. Even in some ways, these technology will replace the SNP array. There are several methods of genotyping based on NGS in the following.
• RRL - Reduced Representation Library
• GBS - Genotyping By Sequencing
• CRoPS - Complexity Reduction of Polymorphic Sequences
• MSG - Multiplex Shotgun Genotyping
• RAD - Restriction site Associated DNA
There are some articles about the above technology:
van Tassell, C. P. et al. SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries. Nature Methods 5, 247–252 (2008).
The first description of the RRL approach using NGS.
Andolfatto, P. et al. Multiplexed shotgun genotyping for rapid and efficient genetic mapping. Genome Res.21, 610–617 (2011).
The original description of MSG, describing the hidden Markov model approach to imputation of genotypes.
van Orsouw, N. J. et al. Complexity reduction of polymorphic sequences (CRoPS): a novel approach for large-scale polymorphism discovery in complex genomes. PLoS ONE 2, e1172 (2007).
The original description of the CRoPS method.
Baird, N. A. et al. Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS ONE 3, e3376 (2008).
The original description of high-throughputRAD-seq.
Elshire, R. J. et al. A robust, simple genotyping‑by‑sequencing (GBS) approach for high diversity species. PLoS ONE 6, e19379 (2011).
The original description of the GBS method.
https://blog.sciencenet.cn/blog-491809-484309.html
上一篇：RRL（reduced represent libraries）
下一篇：CRoPS - Complexity Reduction of Polymorphic Sequences
NGS(next generation sequencing) is very useful for genotyping. In recently or in the future, genotyping based on NGS will become very popular. Even in some ways, these technology will replace the SNP array. There are several methods of genotyping based on NGS in the following.
• RRL - Reduced Representation Library
• GBS - Genotyping By Sequencing
• CRoPS - Complexity Reduction of Polymorphic Sequences
• MSG - Multiplex Shotgun Genotyping
• RAD - Restriction site Associated DNA
There are some articles about the above technology:
van Tassell, C. P. et al. SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries. Nature Methods 5, 247–252 (2008).
The first description of the RRL approach using NGS.
Andolfatto, P. et al. Multiplexed shotgun genotyping for rapid and efficient genetic mapping. Genome Res.21, 610–617 (2011).
The original description of MSG, describing the hidden Markov model approach to imputation of genotypes.
van Orsouw, N. J. et al. Complexity reduction of polymorphic sequences (CRoPS): a novel approach for large-scale polymorphism discovery in complex genomes. PLoS ONE 2, e1172 (2007).
The original description of the CRoPS method.
Baird, N. A. et al. Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS ONE 3, e3376 (2008).
The original description of high-throughputRAD-seq.
Elshire, R. J. et al. A robust, simple genotyping‑by‑sequencing (GBS) approach for high diversity species. PLoS ONE 6, e19379 (2011).
The original description of the GBS method.
https://blog.sciencenet.cn/blog-491809-484309.html
上一篇：RRL（reduced represent libraries）
下一篇：CRoPS - Complexity Reduction of Polymorphic Sequences
