已有 6458 次阅读2010-11-16 12:20|个人分类:未分类|系统分类:科研笔记|tag, RAD
Restriction site Associated DNA (RAD) markers are a type of genetic marker that can be used for genetic mapping.最近经常碰到RAD tag sequencing的问题,发表下看法:这种方法适合于低成本的寻找marker,特别是在大群体当中,具有很好的应用前景。当然,应用测序技术通过sequenced RAD tag 确定并分型SNP非常的好,而且现在很多理论同样显示,这种方法对于没有reference的物种同样适用,无疑这将是非常有利于推广的方法。目前的难点在于如果攻克并对没有reference的问题进行成熟化。
以下是几篇代表作:
^ abcdefg Miller MR, Dunham JP, Amores A, Cresko WA, Johnson EA. (2007) Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA (RAD) markers. Genome Research. 17(2):240-248.
^ abcde Baird NA, Etter PD, Atwood TS, Currey MC, Shiver AL, Lewis ZA, Selker EU, Cresko WA, Johnson EA. (2008) Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS One. 3(10):e3376.
^ ab Lewis ZA, Shiver AL, Stiffler N, Miller MR, Johnson EA, Selker EU. (2007) High density detection of restriction site associated DNA (RAD) markers for rapid mapping of mutated loci in Neurospora. Genetics. 177(2):1163-1171.
^ ab Hohenlohe PA, Bassham S, Etter PD, Stiffler N, Johnson EA, Cresko WA. (2010) Population genomics of parallel adaptation in threespine stickleback using sequenced RAD tags. PLoS Genetics. 6(2):e1000862
RAD tags are the DNA sequences that immediately flank each instance of a particular restriction enzyme site throughout a genome and the process of isolating RAD tags is an important aspect of RAD markers and mapping. Different RAD tag densities can be achieved by using different restriction enzymes during the isolation process.