Salicylic acid carboxyl glucosyltransferase UGT87E7 regulates disease resistance in Camellia Sinensis

第一作者：胡芸青、张孟婷

通讯单位：安徽农业大学

通讯作者：宋传奎

背景回顾：Plant immune response following pathogenic infection is regulated by plant hormones, and salicylic acid (SA) and its sugar conjugates play important roles in establishing basal resistance. 

植物在病原侵染后的免疫反应受植物激素的调节，而水杨酸(SA)及其糖结合物在基础抗性的建立中起着重要作用。

研究内容：Here, the important pathogen Pseudopestalotiopsis camelliae-sinensis (Pcs) was isolated from tea gray blight, one of the most destructive diseases in tea plantations. 

在此，从茶园危害最大的病害之一--茶树轮斑病中分离到重要病原菌--茶树拟青霉。

主要结论：Transcriptomic analysis led to the discovery of the putative Camellia sinensis UDP-glucosyltransferase CsUGT87E7 whose expression was significantly induced by SA application and Pcs infection. Recombinant CsUGT87E7 glucosylates SA with a Km value of 12 μM to form SA glucose ester (SGE). Downregulation reduced the accumulation of SGE, and CsUGT87E7-silenced tea plants exhibited greater susceptibility to pathogen infection than control plants. Similarly, CsUGT87E7-silenced tea leaves accumulated significantly less SA after infection and showed reduced expression of pathogenesis-related genes. These results suggest that CsUGT87E7 is an SA carboxyl glucosyltransferase that plays a positive role in plant disease resistance by modulating SA homeostasis through a mechanism distinct from that described in Arabidopsis (Arabidopsis thaliana).

转录分析发现了可能的茶树UDP-葡萄糖基转移酶CsUGT87E7，其表达受SA处理和PCs感染的显著诱导。重组CsUGT87E7将Km值为12微米的SA葡糖化，形成SA葡萄糖酯(SGE)。下调表达减少了SGE的积累，CsUGT87E7沉默的茶树表现出比对照更敏感的病原菌侵染。同样，CsUGT87E7沉默的茶叶在感染后积累的SA明显减少，病程相关基因的表达减少。这些结果表明，CsUGT87E7是一种SA羧基葡萄糖转移酶，通过与拟南芥不同的机制调节SA的动态平衡，从而在植物抗病中发挥积极的作用。

 结论：This study provides insight into the mechanisms of SA metabolism and highlights the role of SGE in the modulation of plant disease resistance.

该研究对SA代谢的机制进行了深入的研究，并强调了SGE在植物抗病调节中的作用。

全文链接：https://academic.oup.com/plphys/article/188/3/1507/6454116

**宋传奎**

作者简介：

研究方向： 茶树香气物质代谢与生物工程 

2015——至今     茶与食品科技学院  教授

2011/10-2015/9   慕尼黑工业大学         天然产物生物技术  博士；

2009/9-2011/6     北   京   大   学          茶叶生化与健康     硕士连培；

2008/9-2011/6    西北农林科技大学              茶学              硕士；

2004/9-2008/6    西北农林科技大学              园艺              本科；

Journal: Plant Physiology

Published date: December 06, 2021​