Integrated multi-omic data and analyses reveal the pathways underlying key ornamental traits in carnation flowers

第一作者：Xiaoni Zhang

第一单位：华中农业大学

通讯作者：Xiaopeng Fu

 Abstarct 

背景回顾：Carnation (Dianthus caryophyllus) is one of the most popular ornamental flowers in the world. 

康乃馨（Dianthus caryophyllus）是全球最受喜爱的观赏花卉之一。

提出问题：Although numerous studies on carnations exist, the underlying mechanisms of flower color, fragrance, and the formation of double flowers remain unknown. 

尽管对于康乃馨的研究已经很多，但是其花色、花香以及双花的潜在分子机制仍不清楚。

主要研究：Here, we employed an integrated multi-omics approach to elucidate the genetic and biochemical pathways underlying the most important ornamental features of carnation flowers. 

本文中，作者通过多组学的方法阐释了控制康乃馨花重要观赏性状的遗传和生化途径。

结果1-康乃馨基因组：First, we assembled a high-quality chromosome-scale genome (636 Mb with contig N50 as 14.67 Mb) of D. caryophyllus, the ‘Scarlet Queen’. 

首先，作者组装了康乃馨“猩红女王”品种的高质量染色体级别的基因组，contig N50长14.67 Mb，基因组大小636 Mb。

结果2-代谢组鉴定花色素与花香挥发性化学物质的时空模式：Next, a series of metabolomic datasets was generated with a variety of instrumentation types from different parts of the flower at multiple stages of development to assess spatial and temporal differences in the accumulation of pigment and volatile compounds. 

接着，通过对康乃馨花不同组织、不同发育时期的一系列的代谢组数据，评估了康乃馨花色素和挥发性化合物积累的时空特异性。

结果3-结合转录组鉴定控制花色和花香的关键基因：Finally, transcriptomic data were generated to link genomic, biochemical, and morphological patterns to propose a set of pathways by which ornamental traits such as petal coloration, double flowers, and fragrance production are formed. Among them, the transcription factors bHLHs, MYBs, and a WRKY44 homolog are proposed to be important in controlling petal color patterning and genes such as coniferyl alcohol acetyltransferase and eugenol synthase are involved in the synthesis of eugenol.

最后，作者通过关联转录组数据与基因组、生化、形态数据，鉴定了一系列与康乃馨花瓣着色、双花、花香等关键观赏性状相关的通路。其中，bHLHs、MYBs和一个WRKY44同源基因被认为在控制康乃馨花瓣着色模式方面发挥重要作用；另外，松柏醇乙酰转移酶基因和丁香酚合酶基因参与了丁香酚的合成。

结论：The integrated dataset of genomics, transcriptomics, and metabolomics presented herein provides an important foundation for understanding the underlying pathways of flower development and coloration, which in turn can be used for selective breeding and gene editing for the development of novel carnation cultivars.

本文所报道的基因组、转录组和代谢组数据为进一步理解控制康乃馨花发育和着色的分子机制提供了基础，将有助于将来利用选择育种和基因编辑来开发新的康乃馨品种。

** 傅小鹏 **

doi: https://doi.org/10.1111/pbi.13801

Journal: Plant Biotechnology Journal

Published date: March 05, 2022