背景回顾：Pre‐harvest sprouting (PHS), the germination of grain before harvest, is a serious problem resulting in wheat yield and quality losses. 

主要发现：Here, we mapped the PHS resistance gene PHS‐3D from synthetic hexaploid wheat to a 2.4 Mb presence‐absence variation (PAV) region and found that its resistance effect was attributed to the pleiotropic Myb10‐D by integrated omics and functional analyses.

结果1-单倍型：Three haplotypes were detected in this PAV region among 262 worldwide wheat lines and 16 Aegilops tauschii, and the germination percentages of wheat lines containing Myb10‐D was approximately 40% lower than that of the other lines. 

结果2-多组学：Transcriptome and metabolome profiling indicated that Myb10‐D affected the transcription of genes in both the flavonoid and ABA biosynthesis pathways, which resulted in increases in flavonoids and ABA in transgenic wheat lines.

结果3-作用机制：Myb10‐D activates NCED by binding the secondary wall MYB‐ esponsive element (SMRE) to promote ABA biosynthesis in early wheat seed development stages. We revealed that the newly discovered function of Myb10‐D confers PHS resistance by enhancing ABA biosynthesis to delay germination in wheat. 

结论：The PAV harboring Myb10‐D associated with grain color and PHS will be useful for understanding and selecting white grained PHS resistant wheat cultivars.

穗发芽（Pre‐harvest sprouting，PHS）是一种籽粒在成熟前就在穗上发芽的现象，会导致小麦产量和质量的损失。本文中，作者在六倍体小麦中鉴定到了一个PHS抗性位点，位于一个长度2.4 Mb的存在/缺失变异（PAV）区域内，并通过多组学和功能分析发现该位点的抗性作用源自于Myb10‐D的多效性。基于262份全球小麦品系和16份节节麦材料，作者鉴定到了该PAV区域的3个单倍型，并且含有Myb10‐D的小麦品系的萌发率大约会比其它品系低约40%。转录和代谢图谱显示，Myb10‐D会影响类黄酮和ABA生物合成通路相关基因的转录，从而转基因的小麦品系中类黄酮和ABA含量增加。Myb10‐D通过结合次生壁MYB响应元件SMRE激活NCED的表达，从而增强ABA的生物合成，以延迟小麦的萌发。因此，本文鉴定到了一个与籽粒颜色和PHS相关的、含有Myb10‐D的PAV，有助于将来对于白色籽粒PHS抗性小麦栽培种的理解和选择。