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第一作者:Anat Hendelman
第一单位:冷泉港实验室
通讯作者:Zachary B. Lippman
Abstract
背景回顾:Divergence of gene function is a hallmark of evolution, but assessing functional divergence over deep time is not trivial.
提出问题:The few alleles available for cross-species studies often fail to expose the entire functional spectrum of genes, potentially obscuring deeply conserved pleiotropic roles.
主要研究:Here, we explore the functional divergence of WUSCHEL HOMEOBOX9 (WOX9), suggested to have species-specific roles in embryo and inflorescence development.
结果1-顺式调控编辑驱动系统:Using a cis-regulatory editing drive system, we generate a comprehensive allelic series in tomato, which revealed hidden pleiotropic roles for WOX9.
结果2-关键区域鉴定:Analysis of accessible chromatin and conserved cis-regulatory sequences identifies the regions responsible for this pleiotropic activity, the functions of which are conserved in groundcherry, a tomato relative.
结果3-跨远缘物种间的保守性:Mimicking these alleles in Arabidopsis, distantly related to tomato and groundcherry, reveals new inflorescence phenotypes, exposing a deeply conserved pleiotropy.
结论:We suggest that targeted cis-regulatory mutations can uncover conserved gene functions and reduce undesirable effects in crop improvement.
摘 要
基因功能的分化是演化的一个标志,但评估长久的功能分化并非易事。可以用于跨物种研究的少数等位基因,由于可能会掩盖高度保守的多效性功能,往往不能揭示基因的整体功能谱。本文中,作者研究了WOX9基因的功能分化,发现其在胚胎发育和花序发育过程中具有物种特异性的功能。利用顺式调控编辑驱动系统,作者获得了番茄的一个全面等位基因谱,解析了WOX9基因潜在的多效性作用。对于染色质可及性和保守的顺式调控序列的分析,作者鉴定到了WOX9基因功能多效性的关键区域,并且该区域在番茄的近缘物种地樱桃中功能保守。作者进一步选择了一个与番茄和地樱桃亲缘关系比较远的植物拟南芥中,模拟这些等位基因,发现了新的花序表型,揭示了一种高度保守的多效性。作者认为靶向的顺式调控突变能够揭示保守的基因功能,减少作物改良过程中的不良影响。
通讯作者
**Zachary B. Lippman**
个人简介:
2004年,冷泉港实验室,博士。
研究方向:植物开花调控。
doi: https://doi.org/10.1016/j.cell.2021.02.001
Journal: Cell
Published date: Mar 04, 2021
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