A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat

第一作者：Ming Luo

第一单位：澳大利亚CSIRO农业与食品研究所

通讯作者：Michael Ayliffe

 Abstract 

背景回顾：Breeding wheat with durable resistance to the fungal pathogen Puccinia graminis f. sp. tritici (Pgt), a major threat to cereal production, is challenging due to the rapid evolution of pathogen virulence.

解决方案与问题：Increased durability and broad-spectrum resistance can be achieved by introducing more than one resistance gene, but combining numerous unlinked genes by breeding is laborious.

主要研究：Here we generate polygenic Pgt resistance by introducing a transgene cassette of five resistance genes into bread wheat as a single locus and show that at least four of the five genes are functional.

结果1-高抗：These wheat lines are resistant to aggressive and highly virulent Pgt isolates from around the world and show very high levels of resistance in the field.

结果2-单基因遗传：The simple monogenic inheritance of this multigene locus greatly simplifies its use in breeding.

潜在问题：However, a new Pgt isolate with virulence to several genes at this locus suggests gene stacks will need strategic deployment to maintain their effectiveness.

doi: https://doi.org/10.1038/s41587-020-00770-x

Journal: Nature Biotechnology

Published date: Jan 04, 2021