博文

the plant journal：HDA19独立于光周期参与植物开花时间调控

已有 3410 次阅读 2019-3-13 15:42 |个人分类:每日摘要|系统分类:论文交流

The HDA19 histone deacetylase complex is involved in the regulation of flowering time in a photoperiod‐dependent manner

First author: Yong‐Qiang Ning; Affiliations: China Agricultural University (中国农业大学): Beijing, China

Corresponding author: Xin‐Jian He

Chromatin modifications are known to affect flowering time in plants, but little is known about how these modifications regulate flowering time in response to environmental signals like photoperiod. In Arabidopsis thaliana, HDC1, a conserved subunit of the RPD3‐like histone deacetylase (HDAC) complex, was previously reported to regulate flowering time via the same mechanism as does the HDAC HDA6. Here, we demonstrate that HDC1, SNLs and MSI1 are shared subunits of the HDA6 and HDA19 HDAC complexes. While the late‐flowering phenotype of the hda6 mutant is independent of photoperiod, the hda19, hdc1 and snl2/3/4 mutants flower later than or at a similar time to the wild‐ ype in long‐day conditions but flower earlier than the wild‐ ype in short‐day conditions. Our genome‐wide analyses indicate that the effect of hdc1 on histone acetylation and transcription is comparable with that of hda19 but is different from that of hda6. Especially, we demonstrate that the HDA19 complex directly regulates the expression of two flowering repressor genes related to the gibberellin signaling pathway. Thus, the study reveals a photoperiod‐dependent role of the HDA19 HDAC complex in the regulation of flowering time.

染色质修饰会影响植物的开花时间，但这些修饰如何响应于光周期等外界环境信号来调控植物的开花时间还不清楚。在拟南芥中，HDC1是HDAC复合体的一个保守亚基，在之前的报道中该蛋白能够通过和HDAC HDA6一样的途径来调控植物的开花时间。本文中，作者揭示了HDC1、SNLs和MSI1是HDA6和HDA19 HDAC复合物共享的亚基。hda6突变体中的晚花表型与光周期无关，而hda19, hdc1和snl2/3/4突变体开花时间要迟于或者和野生型植物在长日照条件下的开花时间相近，但要早于野生型在短日照条件下的开花时间。本文的基因组范围的分析显示hdc1在组蛋白乙酰化和转录方面的作用与hda19相当，但是与hda6完全不同。尤其是，作者发现HDA19复合物能够直接调控两个开花抑制基因的表达，这两个基因参与赤霉素信号转导通路。因此，本文的研究揭示了HDA19 HDAC复合物独立于光周期作用于植物开花时间调控的分子机制。