miR-378 attenuates muscle regeneration by delaying satellite cell activation and differentiation in mice

Ping Zeng,  Wanhong Han,  Changyin Li,  Hu Li,  Dahai Zhu,  Yong Zhang and  Xiaohong Liu

Division of Geriatrics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China  

Acta Biochim Biophys Sin 2016, 48: 833–839; doi: 10.1093/abbs/gmw077

Skeletal muscle mass and homeostasis during postnatal muscle development and regeneration largely depend on adult muscle stem cells (satellite cells). We recently showed that global overexpression of miR-378 significantly reduced skeletal muscle mass in mice. In the current study, we used miR-378 transgenic (Tg) mice to assess the in vivo functional effects of miR-378 on skeletal muscle growth and regeneration. Cross-sectional analysis of skeletal muscle tissues showed that the number and size of myofibers were significantly lower in miR-378 Tg mice than in wild-type mice. Attenuated cardiotoxin-induced muscle regeneration in miR-378 Tg mice was found to be associated with delayed satellite cell activation and differentiation. Mechanistically, miR-378 was found to directly target Igf1r in muscle cells both in vitro and in vivo. These miR-378 Tg mice may provide a model for investigating the physiological and pathological roles of skeletal muscle in muscle-associated diseases in humans, particularly in sarcopenia.

Delayed muscle regeneration in miR-378 Tg mice due to attenuated satellite cell differentiation

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