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ABBS: TAR cloning of biosynthetic gene cluster

已有 321 次阅读 2019-8-27 16:39 |个人分类:期刊新闻|系统分类:论文交流| TAR cloning, gene cluster |文章来源:转载

TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in Streptomyces aureofaciens

Naxin Wu, He Huang, Taoling Min, and Haifeng Hu

School of Pharmacy, Department of Pharmacology, Fudan University, Shanghai 201203, China

Acta Biochim Biophys Sin 2017, 49: 1129–1134; doi: 10.1093/abbs/gmx110

6-Demethylchlortetracycline (6-DCT), a tetracycline antibiotic produced by Streptomyces aureofaciens, is a crucial precursor employed for the semi-synthesis of tigecycline, minocycline, and amadacyclin (PTK 0796). In this study, the 6-DCT biosynthetic gene cluster (BGC) was cloned from genomic DNA of a high 6-DCT-producing strain, S. aureofaciens DM-1, using the transformation-associated recombination method. An extra copy of the 6-DCT BGC was introduced and integrated into the chromosome of S. aureofaciens DM-1. Duplication of the 6-DCT BGC resulted in a maximum increase of the 6-DCT titer by 34%.


 Direct cloning of the 6-DCT BGC using the TAR method




1 Innovation - TAR cloning: insights into gene function, long-range haplotypes and genome structure and evolution

2 Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast

3 Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology

4 Variation in human chromosome 21 ribosomal RNA genes characterized by TAR cloning and long-read sequencing

Defining the minimal length of sequence homology required for selective gene isolation by TAR cloning

6 Direct cloning of human 10q25 neocentromere DNA using transformation-associated recombination (TAR) in yeast

7 Construction of human chromosome 16- and 5-specific circular YAC/BAC libraries by in vivo recombination in yeast (TAR cloning)

8 Cloning Large Natural Product Gene Clusters from the Environment: Piecing Environmental DNA Gene Clusters Back Together with TAR


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