...that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided... ...genome editing in human cells. NgAgo binds 5′ phosphorylated single-... ...loaded with the gDNA. The NgAgo–gDNA system does not require...
Argonaute (NgAgo) with guide DNA (gDNA... ...for zebrafish. Recently the gDNA/NgAgosystem has elicited much interest... ...we investigated whether the gDNA/NgAgo system could be used to...
(NgAgo) as a genome engineering... ...with a plasmid DNA encoding NgAgo and a 24-nucleotide,... ...gDNA sequences and used the NgAgo vector provided by Gao ...well as expression of the NgAgo protein. Despite various attempts... to optimizeNgAgo-mediated genome editing in three...
...but the authors found that NgAgo, an Argonaute from ...25 nucleotides (nt). WhenNgAgo was expressed in human 293T... ...of off-target binding. Copurified NgAgo and ssDNA guides led to... . Unlike Cas9, NgAgo seemed to randomly remove 1...
(NgAgo). They found that,... ...when expressed in human cells,NgAgo did not associate with endogenous... ...the risk of guide swapping. NgAgo formed double-strand breaks (... ...repair with donor DNA. The NgAgo–gDNA system was at least...
(NgAgo) is a precise and... ...by the Cas9 nuclease — NgAgo can cleave targets using single... ...in length as a guide. NgAgo was found to create site... ...suggesting high fidelity of the NgAgo–gDNA system. Correct insertion...