《中药生物技术》教学进度

教材： 中药生物技术，贾景明，专业教材，化学工业出版社，2008

一，点评HW4

1. 根据底物和产物的结构式确定酶的大类，也可以根据酶的编号。

   需要注意的是合酶synthase与合成酶（即连接酶）synthetase/ligase

2. 参考书

   
   

   生物化学教程。王镜岩等。高等教育出版社。2008

3. 几个酶的大类

   5-磷酸脱氧木-酮糖还原酶1-deoxy-D-xylulose5-phosphate reductoisomerase (DXR; EC 1.1.1.267)，K_M=250 μM for DXP
   

   "Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residues." Kuzuyama T., Takahashi S., Takagi M., Seto H. J. Biol. Chem. 275:19928-19932(2000)

   https://www.uniprot.org/uniprot/P45568

   
   

   色氨酸脱羧酶Tryptophan decarboxylase (TDC; EC 4.1.1.28), a pyridoxal dependent enzyme converts tryptophan to tryptamine.

   栊牛儿醇-10羟化酶geraniol-10-hydroxylase (G10H; EC 2.5.1.1)

   次番木鳖苷合酶secologanin synthase (SLS; EC 1.3.3.9)

   异胡豆苷合酶strictosidine synthase (STR; EC 4.3.3.2)

   "De novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla, a non-model plant with potent anti-alzheimer’s properties" Guo, Q., Ma, X., Wei, S. et al. BMC Genomics (2014) 15: 676. https://doi.org/10.1186/1471-2164-15-676

    

   secologanin synthase EC:1.14.19.62 "Indole alkaloid biosynthesis in Catharanthus roseus: new enzyme activities and identification of cytochrome P450 CYP72A1 as secologanin synthase." Irmler S., Schroeder G., St Pierre B., Crouch N.P., Hotze M., Schmidt J., Strack D., Matern U., Schroeder J. Plant J. 24:797-804(2000)

二，授课

1. 常用资源：

   
   

   https://www.ncbi.nlm.nih.gov/	National Center for Biotechnology   Information
   http://www.sciencenet.cn/bbs/	科学网论坛
   http://www.dxy.cn/	丁香园
   http://www.bioon.com/	生物谷
   http://www.ebiotrade.com/	生物通
   http://www.genome.jp/ http://www.genome.jp/kegg/	KEGG   Atlas Global maps of cell/organism functions  KEGG PATHWAY Pathway maps and pathway   modules  KEGG BRITE Functional hierarchies and   ontologies  KEGG GENES Genomes, genes, proteins, and   orthologs  KEGG LIGAND Chemical compounds, drugs,   glycans, and reactions
   http://www.rcsb.org/pdb/home/home.do	The RCSB PDB provides a variety of tools   and resources for studying the structures of biological macromolecules and   their relationships to sequence, function, and disease.

2. 杂交：Southern  Blotting，Northern Blotting，Western Blotting

3. 分子克隆/基因工程

区别：vector, carrier; selection, screening

三，作业（自由选择）

HW4四个酶的基因：name，ID，length

DXR，G10H，SLS，STR

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