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The Plant Cell:生长素生物合成的转录激活驱动体细胞的发育重编程

已有 685 次阅读 2022-8-6 23:09 |个人分类:每日摘要|系统分类:论文交流

Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells

第一作者Yuki Sakamoto

第一单位东京大学

通讯作者Keiko Sugimoto


 Abstract 

背景回顾Plant cells exhibit remarkable plasticity of their differentiation states, enabling regeneration of whole plants from differentiated somatic cells


提出问题How they revert cell fate and express pluripotency, however, remains unclear. 


主要发现In this study we demonstrate that transcriptional activation of auxin biosynthesis is crucial for reprogramming differentiated Arabidopsis (Arabidopsis thaliana) leaf cells. 


结果1-乙酰化-PLTs-YUC1-生长素生物合成Our data show that interfering with the activity of histone acetyltransferases dramatically reduces callus formation from leaf mesophyll protoplasts. Histone acetylation permits transcriptional activation of PLETHORAs (PLTs), leading to the induction of their downstream YUCCA1 (YUC1) gene encoding an enzyme for auxin biosynthesis.  


结果2-生长素-ARF/IAA-MYB3Rs-细胞周期Auxin biosynthesis is in turn required to accomplish initial cell division through the activation of G2/M phase genes mediated by MYB DOMAIN PROTEIN 3-RELATED (MYB3Rs). We further show that the AUXIN RESPONSE FACTOR 7 (ARF7)/ARF19 and INDOLE-3-ACETIC ACID INDUCIBLE 3 (IAA3)/IAA18-mediated auxin signaling pathway is responsible for cell cycle reactivation by transcriptionally upregulating MYB3R4


结论These findings provide a mechanistic model of how differentiated plant cells revert their fate and reinitiate the cell cycle to become pluripotent. 


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 摘 要 

处于分化状态的植物细胞仍具有超强的可塑性,能够由已分化的体细胞再生出一颗完整的植株。但是,植物体细胞是如何逆转细胞命运,从而获得多能性的,这一点还不清楚。本文中,作者发现生长素生物合成的转录激活对于重编程拟南芥已分化的叶片细胞至关重要。作者发现,干扰组蛋白乙酰转移酶的活性,能够显著减少拟南芥由叶肉原生质体为外植体诱导形成的愈伤。组蛋白乙酰化能够促进PLTs基因的转录激活,从而诱导其下游靶基因YUC1的表达,而YUC1基因编码的酶正是作用于生长素的生物合成。接着,生长素生物合成通过MYB3Rs介导的G2/M期基因激活,完成初始的细胞分裂。作者进一步的研究发现,生长素响应因子ARF7/19和IAA3/18介导的生长素信号转导途径作用于MYB3R4介导的细胞周期重激活。本文的研究结果揭示了一个分子模型,解释了已分化的植物细胞如何逆转自身的发育命运,并起始细胞周期从而获得多能性。




 Keiko Sugimoto 


个人简介:

1989-1993年,国际基督教大学,学士;

1993-1995年,大阪大学,硕士;

1995-1999年,澳大利亚国立大学,博士。


研究方向:在不断变化的环境中,植物如何整合发育和环境信号最大限度地促进器官生长。


doi: https://doi.org/10.1093/plcell/koac218


Journal: The Plant Cell

Published date: August 04, 2022


Cite:
Yuki Sakamoto, Ayako Kawamura, Takamasa Suzuki, Shoji Segami, Masayoshi Maeshima, Stefanie Polyn, Lieven De Veylder, Keiko Sugimoto. Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells. The Plant Cell, 2022. DOI: https://doi.org/10.1093/plcell/koac218​



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